Identification of novel Salmonella enterica Serovar Thyphimurium DT104-specific prophage and nonprophage chromosomal sequences among serovar Thyphimurium isolates by genomic subtractive hybridization

A.P.H.M. Hermans, T. Abee, M.H. Zwietering, H.J.M. Aarts

Research output: Contribution to journalArticleAcademicpeer-review

25 Citations (Scopus)

Abstract

Genomic subtractive hybridization was performed between Salmonella enterica serovar Typhimurium LT2 and DT104 to search for novel Salmonella serovar Typhimurium DT104-specific sequences. The subtraction resulted mainly in the isolation of DNA fragments with sequence similarity to phages. Two fragments identified were associated with possible virulence factors. One fragment was identical to irsA of Salmonella serovar Typhimurium ATCC 14028, which is suggested to be involved in macrophage survival. The other fragment was homologous to HldD, an Escherichia coli O157:H7 lipopolysaccharide assembly-related protein. Five selected DNA fragments¿irsA, the HldD homologue, and three fragments with sequence similarity to prophages¿were tested for their presence in 17 Salmonella serovar Typhimurium DT104 isolates and 27 non-DT104 isolates by PCR. All five selected DNA fragments were Salmonella serovar Typhimurium DT104 specific among the serovar Typhimurium isolates tested. These DNA fragments can be useful for better detection and typing of Salmonella serovar Typhimurium DT104
Original languageEnglish
Pages (from-to)4979-4985
JournalApplied and Environmental Microbiology
Volume71
Issue number9
DOIs
Publication statusPublished - 2005

Keywords

  • multiple-drug resistance
  • escherichia-coli
  • molecular characterization
  • fimbrial operon
  • manno-heptose
  • phage types
  • dt104
  • serotype
  • bacteriophage
  • epidemic

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