Identification of new populations of chicken natural killer (NK) cells

Christine A. Jansen; Peter M. van de Haar; Daphne van Haarlem; Peter van Kooten; Sjaak de Wit; Willem van Eden; Birgit C. Viertlböck; Thomas W. Göbel; Lonneke Vervelde

doi:10.1016/j.dci.2010.02.009

Identification of new populations of chicken natural killer (NK) cells

Christine A. Jansen^*, Peter M. van de Haar, Daphne van Haarlem, Peter van Kooten, Sjaak de Wit, Willem van Eden, Birgit C. Viertlböck, Thomas W. Göbel, Lonneke Vervelde

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

59 Citations (Scopus)

Abstract

Natural killer (NK) cell activity is conserved throughout vertebrate development, but characterization of non-mammalian NK-cells has been hampered by the absence of specific mAbs for these cells.Monoclonal antibodies were generated against in vitro IL-2 expanded sorted CD3-CD8α+ peripheral blood lymphocytes, previously described to contain chicken NK-cells. Screening of embryonic and adult splenocytes with hybridoma supernatants resulted in five candidate NK markers.Activation of chicken NK-cells with PMA/Ionomycin or with the NK target cell-line LSCC-RP9 resulted in increased expression of CD107 (LAMP-1) and a newly developed flow cytometry based cytotoxicity assay showed that NK-cells were able to kill target cells. Combining NK markers with functional assays indicated that marker positive cells showed NK-cell function.In conclusion, we generated new monoclonal antibodies and developed two functional assays which will enhance our understanding of the role of NK-cells in healthy and diseased chickens.

Original language	English
Pages (from-to)	759-767
Number of pages	9
Journal	Developmental and Comparative Immunology
Volume	34
Issue number	7
DOIs	https://doi.org/10.1016/j.dci.2010.02.009
Publication status	Published - Jul 2010
Externally published	Yes

Keywords

CD107
Chickens
Markers
NK-cells

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10.1016/j.dci.2010.02.009

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@article{126018dc70514e6dbd085920db665520,

title = "Identification of new populations of chicken natural killer (NK) cells",

abstract = "Natural killer (NK) cell activity is conserved throughout vertebrate development, but characterization of non-mammalian NK-cells has been hampered by the absence of specific mAbs for these cells.Monoclonal antibodies were generated against in vitro IL-2 expanded sorted CD3-CD8α+ peripheral blood lymphocytes, previously described to contain chicken NK-cells. Screening of embryonic and adult splenocytes with hybridoma supernatants resulted in five candidate NK markers.Activation of chicken NK-cells with PMA/Ionomycin or with the NK target cell-line LSCC-RP9 resulted in increased expression of CD107 (LAMP-1) and a newly developed flow cytometry based cytotoxicity assay showed that NK-cells were able to kill target cells. Combining NK markers with functional assays indicated that marker positive cells showed NK-cell function.In conclusion, we generated new monoclonal antibodies and developed two functional assays which will enhance our understanding of the role of NK-cells in healthy and diseased chickens.",

keywords = "CD107, Chickens, Markers, NK-cells",

author = "Jansen, {Christine A.} and {van de Haar}, {Peter M.} and {van Haarlem}, Daphne and {van Kooten}, Peter and {de Wit}, Sjaak and {van Eden}, Willem and Viertlb{\"o}ck, {Birgit C.} and G{\"o}bel, {Thomas W.} and Lonneke Vervelde",

year = "2010",

month = jul,

doi = "10.1016/j.dci.2010.02.009",

language = "English",

volume = "34",

pages = "759--767",

journal = "Developmental and Comparative Immunology",

issn = "0145-305X",

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T1 - Identification of new populations of chicken natural killer (NK) cells

AU - Jansen, Christine A.

AU - van de Haar, Peter M.

AU - van Haarlem, Daphne

AU - van Kooten, Peter

AU - de Wit, Sjaak

AU - van Eden, Willem

AU - Viertlböck, Birgit C.

AU - Göbel, Thomas W.

AU - Vervelde, Lonneke

PY - 2010/7

Y1 - 2010/7

N2 - Natural killer (NK) cell activity is conserved throughout vertebrate development, but characterization of non-mammalian NK-cells has been hampered by the absence of specific mAbs for these cells.Monoclonal antibodies were generated against in vitro IL-2 expanded sorted CD3-CD8α+ peripheral blood lymphocytes, previously described to contain chicken NK-cells. Screening of embryonic and adult splenocytes with hybridoma supernatants resulted in five candidate NK markers.Activation of chicken NK-cells with PMA/Ionomycin or with the NK target cell-line LSCC-RP9 resulted in increased expression of CD107 (LAMP-1) and a newly developed flow cytometry based cytotoxicity assay showed that NK-cells were able to kill target cells. Combining NK markers with functional assays indicated that marker positive cells showed NK-cell function.In conclusion, we generated new monoclonal antibodies and developed two functional assays which will enhance our understanding of the role of NK-cells in healthy and diseased chickens.

AB - Natural killer (NK) cell activity is conserved throughout vertebrate development, but characterization of non-mammalian NK-cells has been hampered by the absence of specific mAbs for these cells.Monoclonal antibodies were generated against in vitro IL-2 expanded sorted CD3-CD8α+ peripheral blood lymphocytes, previously described to contain chicken NK-cells. Screening of embryonic and adult splenocytes with hybridoma supernatants resulted in five candidate NK markers.Activation of chicken NK-cells with PMA/Ionomycin or with the NK target cell-line LSCC-RP9 resulted in increased expression of CD107 (LAMP-1) and a newly developed flow cytometry based cytotoxicity assay showed that NK-cells were able to kill target cells. Combining NK markers with functional assays indicated that marker positive cells showed NK-cell function.In conclusion, we generated new monoclonal antibodies and developed two functional assays which will enhance our understanding of the role of NK-cells in healthy and diseased chickens.

KW - CD107

KW - Chickens

KW - Markers

KW - NK-cells

U2 - 10.1016/j.dci.2010.02.009

DO - 10.1016/j.dci.2010.02.009

M3 - Article

C2 - 20188123

AN - SCOPUS:77951499381

SN - 0145-305X

VL - 34

SP - 759

EP - 767

JO - Developmental and Comparative Immunology

JF - Developmental and Comparative Immunology

IS - 7

ER -

Identification of new populations of chicken natural killer (NK) cells

Abstract

Keywords

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