Identification of new antigen candidates for the early diagnosis of Mycobacterium avium subsp. paratuberculosis infection in goats

Armel Souriau, Sandrine Freret, Benjamin Foret, Peter T.J. Willemsen, Douwe Bakker, Laurence A. Guilloteau*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Currently Mycobacterium avium subsp. paratuberculosis (MAP) infection is diagnosed through indirect tests based on the immune response induced by the infection. The antigens commonly used in IFN-γ release assays (IGRA) are purified protein derivative tuberculins (PPD). However, PPDs, lack both specificity (Sp) and sensitivity (Se) in the early phase of infection. This study investigated the potential of 16 MAP recombinant proteins and five lipids to elicit the release of IFN-γ in goats from herds with or without a history of paratuberculosis. Ten recombinant proteins were selected as potential candidates for the detection of MAP infection in young goats. They were found to detect 25 to 75% of infected shedder (IS) and infected non-shedder (INS) kids younger than 10 months of age. In comparison, PPD was shown to detect only 10% of INS and no IS kids. For seven antigens, Se (21–33%) and Sp (≥ 90%) of IGRA were shown to be comparable with PPD at 20 months old. Only three antigens were suitable candidates to detect IS adult goats, although Se was lower than that obtained with PPD. In paratuberculosis-free herds, IGRA results were negative in 97% of indoor goats and 86% of outdoor goats using the 10 antigens. However, 22 to 44% of one-year-old outdoor goats were positive suggesting that they may be infected. In conclusion, this study showed that ten MAP recombinant proteins are potential candidates for early detection of MAP infected goats. Combining these antigens could form a possible set of MAP antigens to optimize the Se of caprine IGRA.

Original languageEnglish
Pages (from-to)278-287
JournalResearch in Veterinary Science
Volume115
DOIs
Publication statusPublished - 2017

Fingerprint

Paratuberculosis
Mycobacterium avium
Mycobacterium avium subsp. paratuberculosis
early diagnosis
Goats
Early Diagnosis
Tuberculin
goats
antigens
Antigens
tuberculin
Infection
infection
Recombinant Proteins
chemical derivatives
recombinant proteins
paratuberculosis
kids (goats)
proteins
herds

Keywords

  • Antigen
  • Early diagnosis
  • Goat
  • IFN-γ
  • Johne's disease
  • Mycobacterium avium subsp. paratuberculosis
  • Paratuberculosis

Cite this

Souriau, Armel ; Freret, Sandrine ; Foret, Benjamin ; Willemsen, Peter T.J. ; Bakker, Douwe ; Guilloteau, Laurence A. / Identification of new antigen candidates for the early diagnosis of Mycobacterium avium subsp. paratuberculosis infection in goats. In: Research in Veterinary Science. 2017 ; Vol. 115. pp. 278-287.
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title = "Identification of new antigen candidates for the early diagnosis of Mycobacterium avium subsp. paratuberculosis infection in goats",
abstract = "Currently Mycobacterium avium subsp. paratuberculosis (MAP) infection is diagnosed through indirect tests based on the immune response induced by the infection. The antigens commonly used in IFN-γ release assays (IGRA) are purified protein derivative tuberculins (PPD). However, PPDs, lack both specificity (Sp) and sensitivity (Se) in the early phase of infection. This study investigated the potential of 16 MAP recombinant proteins and five lipids to elicit the release of IFN-γ in goats from herds with or without a history of paratuberculosis. Ten recombinant proteins were selected as potential candidates for the detection of MAP infection in young goats. They were found to detect 25 to 75{\%} of infected shedder (IS) and infected non-shedder (INS) kids younger than 10 months of age. In comparison, PPD was shown to detect only 10{\%} of INS and no IS kids. For seven antigens, Se (21–33{\%}) and Sp (≥ 90{\%}) of IGRA were shown to be comparable with PPD at 20 months old. Only three antigens were suitable candidates to detect IS adult goats, although Se was lower than that obtained with PPD. In paratuberculosis-free herds, IGRA results were negative in 97{\%} of indoor goats and 86{\%} of outdoor goats using the 10 antigens. However, 22 to 44{\%} of one-year-old outdoor goats were positive suggesting that they may be infected. In conclusion, this study showed that ten MAP recombinant proteins are potential candidates for early detection of MAP infected goats. Combining these antigens could form a possible set of MAP antigens to optimize the Se of caprine IGRA.",
keywords = "Antigen, Early diagnosis, Goat, IFN-γ, Johne's disease, Mycobacterium avium subsp. paratuberculosis, Paratuberculosis",
author = "Armel Souriau and Sandrine Freret and Benjamin Foret and Willemsen, {Peter T.J.} and Douwe Bakker and Guilloteau, {Laurence A.}",
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language = "English",
volume = "115",
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journal = "Research in Veterinary Science",
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Identification of new antigen candidates for the early diagnosis of Mycobacterium avium subsp. paratuberculosis infection in goats. / Souriau, Armel; Freret, Sandrine; Foret, Benjamin; Willemsen, Peter T.J.; Bakker, Douwe; Guilloteau, Laurence A.

In: Research in Veterinary Science, Vol. 115, 2017, p. 278-287.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Identification of new antigen candidates for the early diagnosis of Mycobacterium avium subsp. paratuberculosis infection in goats

AU - Souriau, Armel

AU - Freret, Sandrine

AU - Foret, Benjamin

AU - Willemsen, Peter T.J.

AU - Bakker, Douwe

AU - Guilloteau, Laurence A.

PY - 2017

Y1 - 2017

N2 - Currently Mycobacterium avium subsp. paratuberculosis (MAP) infection is diagnosed through indirect tests based on the immune response induced by the infection. The antigens commonly used in IFN-γ release assays (IGRA) are purified protein derivative tuberculins (PPD). However, PPDs, lack both specificity (Sp) and sensitivity (Se) in the early phase of infection. This study investigated the potential of 16 MAP recombinant proteins and five lipids to elicit the release of IFN-γ in goats from herds with or without a history of paratuberculosis. Ten recombinant proteins were selected as potential candidates for the detection of MAP infection in young goats. They were found to detect 25 to 75% of infected shedder (IS) and infected non-shedder (INS) kids younger than 10 months of age. In comparison, PPD was shown to detect only 10% of INS and no IS kids. For seven antigens, Se (21–33%) and Sp (≥ 90%) of IGRA were shown to be comparable with PPD at 20 months old. Only three antigens were suitable candidates to detect IS adult goats, although Se was lower than that obtained with PPD. In paratuberculosis-free herds, IGRA results were negative in 97% of indoor goats and 86% of outdoor goats using the 10 antigens. However, 22 to 44% of one-year-old outdoor goats were positive suggesting that they may be infected. In conclusion, this study showed that ten MAP recombinant proteins are potential candidates for early detection of MAP infected goats. Combining these antigens could form a possible set of MAP antigens to optimize the Se of caprine IGRA.

AB - Currently Mycobacterium avium subsp. paratuberculosis (MAP) infection is diagnosed through indirect tests based on the immune response induced by the infection. The antigens commonly used in IFN-γ release assays (IGRA) are purified protein derivative tuberculins (PPD). However, PPDs, lack both specificity (Sp) and sensitivity (Se) in the early phase of infection. This study investigated the potential of 16 MAP recombinant proteins and five lipids to elicit the release of IFN-γ in goats from herds with or without a history of paratuberculosis. Ten recombinant proteins were selected as potential candidates for the detection of MAP infection in young goats. They were found to detect 25 to 75% of infected shedder (IS) and infected non-shedder (INS) kids younger than 10 months of age. In comparison, PPD was shown to detect only 10% of INS and no IS kids. For seven antigens, Se (21–33%) and Sp (≥ 90%) of IGRA were shown to be comparable with PPD at 20 months old. Only three antigens were suitable candidates to detect IS adult goats, although Se was lower than that obtained with PPD. In paratuberculosis-free herds, IGRA results were negative in 97% of indoor goats and 86% of outdoor goats using the 10 antigens. However, 22 to 44% of one-year-old outdoor goats were positive suggesting that they may be infected. In conclusion, this study showed that ten MAP recombinant proteins are potential candidates for early detection of MAP infected goats. Combining these antigens could form a possible set of MAP antigens to optimize the Se of caprine IGRA.

KW - Antigen

KW - Early diagnosis

KW - Goat

KW - IFN-γ

KW - Johne's disease

KW - Mycobacterium avium subsp. paratuberculosis

KW - Paratuberculosis

U2 - 10.1016/j.rvsc.2017.05.025

DO - 10.1016/j.rvsc.2017.05.025

M3 - Article

VL - 115

SP - 278

EP - 287

JO - Research in Veterinary Science

JF - Research in Veterinary Science

SN - 0034-5288

ER -