Abstract
Tomato production is threatened by climate change. High temperatures lead to a decrease of fruit set which correlates with a decrease of pollen fertility. The low viability of tomato pollen under heat stress was previously shown to be associated with alterations in specific metabolites. In this thesis, we used untargeted metabolomics approaches to broaden the identification of metabolites affected by heat stress. We assessed the suitability of pollen isolation methods for metabolomics analysis and considered the pitfalls for our further analysis. We explored the developmental metabolomes of pollen and anthers of different tomato genotypes under control and high temperature conditions and identified that microsporogenesis is a critical developmental stage for the production of mature and fertile pollen grain under heat stress. Several metabolites were putatively associated with tolerance to high temperature such as specific flavonoids, polyamines and alkaloids. These metabolites can be further used as markers in breeding programs to develop new genotypes tolerant to high temperatures.
| Original language | English |
|---|---|
| Qualification | Doctor of Philosophy |
| Awarding Institution |
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| Supervisors/Advisors |
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| Award date | 12 Jun 2017 |
| Place of Publication | Wageningen |
| Publisher | |
| Print ISBNs | 9789463431842 |
| DOIs | |
| Publication status | Published - 2017 |
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Keywords
- solanum lycopersicum
- tomatoes
- genotypes
- heat stress
- heat tolerance
- pollen
- metabolomes
- metabolites
- metabolomics
Cite this
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Identification of metabolites involved in heat stress response in different tomato genotypes. / Paupière, Marine J.
Wageningen : Wageningen University, 2017. 168 p.Research output: Thesis › internal PhD, WU › Academic
TY - THES
T1 - Identification of metabolites involved in heat stress response in different tomato genotypes
AU - Paupière, Marine J.
N1 - WU thesis 6676 Includes bibliographical references. - With summary in English
PY - 2017
Y1 - 2017
N2 - Tomato production is threatened by climate change. High temperatures lead to a decrease of fruit set which correlates with a decrease of pollen fertility. The low viability of tomato pollen under heat stress was previously shown to be associated with alterations in specific metabolites. In this thesis, we used untargeted metabolomics approaches to broaden the identification of metabolites affected by heat stress. We assessed the suitability of pollen isolation methods for metabolomics analysis and considered the pitfalls for our further analysis. We explored the developmental metabolomes of pollen and anthers of different tomato genotypes under control and high temperature conditions and identified that microsporogenesis is a critical developmental stage for the production of mature and fertile pollen grain under heat stress. Several metabolites were putatively associated with tolerance to high temperature such as specific flavonoids, polyamines and alkaloids. These metabolites can be further used as markers in breeding programs to develop new genotypes tolerant to high temperatures.
AB - Tomato production is threatened by climate change. High temperatures lead to a decrease of fruit set which correlates with a decrease of pollen fertility. The low viability of tomato pollen under heat stress was previously shown to be associated with alterations in specific metabolites. In this thesis, we used untargeted metabolomics approaches to broaden the identification of metabolites affected by heat stress. We assessed the suitability of pollen isolation methods for metabolomics analysis and considered the pitfalls for our further analysis. We explored the developmental metabolomes of pollen and anthers of different tomato genotypes under control and high temperature conditions and identified that microsporogenesis is a critical developmental stage for the production of mature and fertile pollen grain under heat stress. Several metabolites were putatively associated with tolerance to high temperature such as specific flavonoids, polyamines and alkaloids. These metabolites can be further used as markers in breeding programs to develop new genotypes tolerant to high temperatures.
KW - solanum lycopersicum
KW - tomatoes
KW - genotypes
KW - heat stress
KW - heat tolerance
KW - pollen
KW - metabolomes
KW - metabolites
KW - metabolomics
KW - solanum lycopersicum
KW - tomaten
KW - genotypen
KW - warmtestress
KW - hittetolerantie
KW - stuifmeel
KW - metabolomen
KW - metabolieten
KW - metabolomica
U2 - 10.18174/413346
DO - 10.18174/413346
M3 - internal PhD, WU
SN - 9789463431842
PB - Wageningen University
CY - Wageningen
ER -