Identification of metabolites involved in heat stress response in different tomato genotypes

Marine J. Paupière

Research output: Thesisinternal PhD, WUAcademic

Abstract

Tomato production is threatened by climate change. High temperatures lead to a decrease of fruit set which correlates with a decrease of pollen fertility. The low viability of tomato pollen under heat stress was previously shown to be associated with alterations in specific metabolites. In this thesis, we used untargeted metabolomics approaches to broaden the identification of metabolites affected by heat stress. We assessed the suitability of pollen isolation methods for metabolomics analysis and considered the pitfalls for our further analysis. We explored the developmental metabolomes of pollen and anthers of different tomato genotypes under control and high temperature conditions and identified that microsporogenesis is a critical developmental stage for the production of mature and fertile pollen grain under heat stress. Several metabolites were putatively associated with tolerance to high temperature such as specific flavonoids, polyamines and alkaloids. These metabolites can be further used as markers in breeding programs to develop new genotypes tolerant to high temperatures.

Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • Visser, Richard, Promotor
  • Bovy, Arnaud, Co-promotor
  • Tikunov, Yury, Co-promotor
Award date12 Jun 2017
Place of PublicationWageningen
Publisher
Print ISBNs9789463431842
DOIs
Publication statusPublished - 2017

Fingerprint

tomatoes
pollen
metabolites
genotype
heat stress
metabolomics
temperature
metabolome
microsporogenesis
isolation techniques
polyamines
fruit set
anthers
alkaloids
flavonoids
heat shock response
viability
climate change
developmental stages
breeding

Keywords

  • solanum lycopersicum
  • tomatoes
  • genotypes
  • heat stress
  • heat tolerance
  • pollen
  • metabolomes
  • metabolites
  • metabolomics

Cite this

Paupière, Marine J.. / Identification of metabolites involved in heat stress response in different tomato genotypes. Wageningen : Wageningen University, 2017. 168 p.
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abstract = "Tomato production is threatened by climate change. High temperatures lead to a decrease of fruit set which correlates with a decrease of pollen fertility. The low viability of tomato pollen under heat stress was previously shown to be associated with alterations in specific metabolites. In this thesis, we used untargeted metabolomics approaches to broaden the identification of metabolites affected by heat stress. We assessed the suitability of pollen isolation methods for metabolomics analysis and considered the pitfalls for our further analysis. We explored the developmental metabolomes of pollen and anthers of different tomato genotypes under control and high temperature conditions and identified that microsporogenesis is a critical developmental stage for the production of mature and fertile pollen grain under heat stress. Several metabolites were putatively associated with tolerance to high temperature such as specific flavonoids, polyamines and alkaloids. These metabolites can be further used as markers in breeding programs to develop new genotypes tolerant to high temperatures.",
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Paupière, MJ 2017, 'Identification of metabolites involved in heat stress response in different tomato genotypes', Doctor of Philosophy, Wageningen University, Wageningen. https://doi.org/10.18174/413346

Identification of metabolites involved in heat stress response in different tomato genotypes. / Paupière, Marine J.

Wageningen : Wageningen University, 2017. 168 p.

Research output: Thesisinternal PhD, WUAcademic

TY - THES

T1 - Identification of metabolites involved in heat stress response in different tomato genotypes

AU - Paupière, Marine J.

N1 - WU thesis 6676 Includes bibliographical references. - With summary in English

PY - 2017

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N2 - Tomato production is threatened by climate change. High temperatures lead to a decrease of fruit set which correlates with a decrease of pollen fertility. The low viability of tomato pollen under heat stress was previously shown to be associated with alterations in specific metabolites. In this thesis, we used untargeted metabolomics approaches to broaden the identification of metabolites affected by heat stress. We assessed the suitability of pollen isolation methods for metabolomics analysis and considered the pitfalls for our further analysis. We explored the developmental metabolomes of pollen and anthers of different tomato genotypes under control and high temperature conditions and identified that microsporogenesis is a critical developmental stage for the production of mature and fertile pollen grain under heat stress. Several metabolites were putatively associated with tolerance to high temperature such as specific flavonoids, polyamines and alkaloids. These metabolites can be further used as markers in breeding programs to develop new genotypes tolerant to high temperatures.

AB - Tomato production is threatened by climate change. High temperatures lead to a decrease of fruit set which correlates with a decrease of pollen fertility. The low viability of tomato pollen under heat stress was previously shown to be associated with alterations in specific metabolites. In this thesis, we used untargeted metabolomics approaches to broaden the identification of metabolites affected by heat stress. We assessed the suitability of pollen isolation methods for metabolomics analysis and considered the pitfalls for our further analysis. We explored the developmental metabolomes of pollen and anthers of different tomato genotypes under control and high temperature conditions and identified that microsporogenesis is a critical developmental stage for the production of mature and fertile pollen grain under heat stress. Several metabolites were putatively associated with tolerance to high temperature such as specific flavonoids, polyamines and alkaloids. These metabolites can be further used as markers in breeding programs to develop new genotypes tolerant to high temperatures.

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KW - genotypes

KW - heat stress

KW - heat tolerance

KW - pollen

KW - metabolomes

KW - metabolites

KW - metabolomics

KW - solanum lycopersicum

KW - tomaten

KW - genotypen

KW - warmtestress

KW - hittetolerantie

KW - stuifmeel

KW - metabolomen

KW - metabolieten

KW - metabolomica

U2 - 10.18174/413346

DO - 10.18174/413346

M3 - internal PhD, WU

SN - 9789463431842

PB - Wageningen University

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