Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus

Gerrit Koop*, Manouk Vrieling, Daniel M.L. Storisteanu, Laurence S.C. Lok, Tom Monie, Glenn Van Wigcheren, Claire Raisen, Xiaoliang Ba, Nicholas Gleadall, Nazreen Hadjirin, Jaap A. Wagenaar

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

22 Citations (Scopus)

Abstract

Bicomponent pore-forming leukocidins are a family of potent toxins secreted by Staphylococcus aureus, which target white blood cells preferentially and consist of an S- and an F-component. The S-component recognizes a receptor on the host cell, enabling high-affinity binding to the cell surface, after which the toxins form a pore that penetrates the cell lipid bilayer. Until now, six different leukocidins have been described, some of which are host and cell specific. Here, we identify and characterise a novel S. aureus leukocidin; LukPQ. LukPQ is encoded on a 45 kb prophage (Î ▪Saeq1) found in six different clonal lineages, almost exclusively in strains cultured from equids. We show that LukPQ is a potent and specific killer of equine neutrophils and identify equine-CXCRA and CXCR2 as its target receptors. Although the S-component (LukP) is highly similar to the S-component of LukED, the species specificity of LukPQ and LukED differs. By forming non-canonical toxin pairs, we identify that the F-component contributes to the observed host tropism of LukPQ, thereby challenging the current paradigm that leukocidin specificity is driven solely by the S-component.

Original languageEnglish
Article number40660
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 2017

Fingerprint Dive into the research topics of 'Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus'. Together they form a unique fingerprint.

  • Cite this

    Koop, G., Vrieling, M., Storisteanu, D. M. L., Lok, L. S. C., Monie, T., Van Wigcheren, G., Raisen, C., Ba, X., Gleadall, N., Hadjirin, N., & Wagenaar, J. A. (2017). Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus. Scientific Reports, 7, [40660]. https://doi.org/10.1038/srep40660