Identification of loci affecting accumulation of secondary metabolites in tomato fruit of a Solanum lycopersicum × Solanum chmielewskii introgression line population

A.R. Ballester Frutos, Yury Tikunov, Jos Molthoff, Silvana Grandillo, Marcela Viquez-Zamora, Ric de Vos, Ruud A. de Maagd, Sjaak van Heusden, Arnaud G. Bovy*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)

Abstract

Semi-polar metabolites such as flavonoids, phenolic acids, and alkaloids are very important health-related compounds in tomato. As a first step to identify genes responsible for the synthesis of semi-polar metabolites, quantitative trait loci (QTLs) that influence the semi-polar metabolite content in red-ripe tomato fruit were identified, by characterizing fruits of a population of introgression lines (ILs) derived from a cross between the cultivated tomato Solanum lycopersicum and the wild species Solanum chmielewskii. By analyzing fruits of plants grown at two different locations, we were able to identify robust metabolite QTLs for changes in phenylpropanoid glycoconjugation on chromosome 9, for accumulation of flavonol glycosides on chromosome 5, and for alkaloids on chromosome 7. To further characterize the QTLs we used a combination of genome sequencing, transcriptomics and targeted metabolomics to identify candidate key genes underlying the observed metabolic variation.

Original languageEnglish
Article number1428
JournalFrontiers in Plant Science
Volume7
DOIs
Publication statusPublished - 2016

Fingerprint

Solanum chmielewskii
Solanum lycopersicum
introgression
secondary metabolites
tomatoes
metabolites
quantitative trait loci
loci
fruits
chromosomes
alkaloids
metabolomics
phenylpropanoids
flavonols
transcriptomics
phenolic acids
glycosides
flavonoids
genes
synthesis

Keywords

  • Alkaloids
  • Flavonoids
  • Introgression lines
  • QTL analysis
  • Tomato (Solanum lycopersicum)

Cite this

@article{21ef0052642f465ba2e72322d221565a,
title = "Identification of loci affecting accumulation of secondary metabolites in tomato fruit of a Solanum lycopersicum × Solanum chmielewskii introgression line population",
abstract = "Semi-polar metabolites such as flavonoids, phenolic acids, and alkaloids are very important health-related compounds in tomato. As a first step to identify genes responsible for the synthesis of semi-polar metabolites, quantitative trait loci (QTLs) that influence the semi-polar metabolite content in red-ripe tomato fruit were identified, by characterizing fruits of a population of introgression lines (ILs) derived from a cross between the cultivated tomato Solanum lycopersicum and the wild species Solanum chmielewskii. By analyzing fruits of plants grown at two different locations, we were able to identify robust metabolite QTLs for changes in phenylpropanoid glycoconjugation on chromosome 9, for accumulation of flavonol glycosides on chromosome 5, and for alkaloids on chromosome 7. To further characterize the QTLs we used a combination of genome sequencing, transcriptomics and targeted metabolomics to identify candidate key genes underlying the observed metabolic variation.",
keywords = "Alkaloids, Flavonoids, Introgression lines, QTL analysis, Tomato (Solanum lycopersicum)",
author = "{Ballester Frutos}, A.R. and Yury Tikunov and Jos Molthoff and Silvana Grandillo and Marcela Viquez-Zamora and {de Vos}, Ric and {de Maagd}, {Ruud A.} and {van Heusden}, Sjaak and Bovy, {Arnaud G.}",
year = "2016",
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language = "English",
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journal = "Frontiers in Plant Science",
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TY - JOUR

T1 - Identification of loci affecting accumulation of secondary metabolites in tomato fruit of a Solanum lycopersicum × Solanum chmielewskii introgression line population

AU - Ballester Frutos, A.R.

AU - Tikunov, Yury

AU - Molthoff, Jos

AU - Grandillo, Silvana

AU - Viquez-Zamora, Marcela

AU - de Vos, Ric

AU - de Maagd, Ruud A.

AU - van Heusden, Sjaak

AU - Bovy, Arnaud G.

PY - 2016

Y1 - 2016

N2 - Semi-polar metabolites such as flavonoids, phenolic acids, and alkaloids are very important health-related compounds in tomato. As a first step to identify genes responsible for the synthesis of semi-polar metabolites, quantitative trait loci (QTLs) that influence the semi-polar metabolite content in red-ripe tomato fruit were identified, by characterizing fruits of a population of introgression lines (ILs) derived from a cross between the cultivated tomato Solanum lycopersicum and the wild species Solanum chmielewskii. By analyzing fruits of plants grown at two different locations, we were able to identify robust metabolite QTLs for changes in phenylpropanoid glycoconjugation on chromosome 9, for accumulation of flavonol glycosides on chromosome 5, and for alkaloids on chromosome 7. To further characterize the QTLs we used a combination of genome sequencing, transcriptomics and targeted metabolomics to identify candidate key genes underlying the observed metabolic variation.

AB - Semi-polar metabolites such as flavonoids, phenolic acids, and alkaloids are very important health-related compounds in tomato. As a first step to identify genes responsible for the synthesis of semi-polar metabolites, quantitative trait loci (QTLs) that influence the semi-polar metabolite content in red-ripe tomato fruit were identified, by characterizing fruits of a population of introgression lines (ILs) derived from a cross between the cultivated tomato Solanum lycopersicum and the wild species Solanum chmielewskii. By analyzing fruits of plants grown at two different locations, we were able to identify robust metabolite QTLs for changes in phenylpropanoid glycoconjugation on chromosome 9, for accumulation of flavonol glycosides on chromosome 5, and for alkaloids on chromosome 7. To further characterize the QTLs we used a combination of genome sequencing, transcriptomics and targeted metabolomics to identify candidate key genes underlying the observed metabolic variation.

KW - Alkaloids

KW - Flavonoids

KW - Introgression lines

KW - QTL analysis

KW - Tomato (Solanum lycopersicum)

U2 - 10.3389/fpls.2016.01428

DO - 10.3389/fpls.2016.01428

M3 - Article

VL - 7

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

M1 - 1428

ER -