Identification of lipid synthesis and secretion proteins in bovine milk

J. Lu, A.C.M. van Hooijdonk, S. Boeren, J. Vervoort, K.A. Hettinga

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Scopus)

Abstract

Lactation physiology is a process that is only partly understood. Proteomics techniques have shown to be useful to help advance the knowledge on lactation physiology in human and rodent species but have not been used as major tools for dairy cows, except for mastitis. In this paper, advanced non-targeted proteomics techniques (Filter aided sample preparation and NanoLC-Orbitrap-MS/MS) were applied to study the milk fat globule membrane and milk serum fraction, resulting in the identification of 246 proteins. Of these, 23 transporters and enzymes were related to lipid synthesis and secretion in mammary gland and their functions are discussed in detail. The identification of these intracellular transporters and enzymes in milk provides a possibility of using milk itself to study lipid synthesis and secretion pathways. This full-scale scan of milk proteins by using non-targeted proteomic analysis helps to reveal the important proteins involved in lipid synthesis and secretion for further examination in targeted studies.
LanguageEnglish
Pages65-72
JournalJournal of Dairy Research
Volume81
Issue number1
DOIs
Publication statusPublished - 2014

Fingerprint

protein secretion
Proteomics
proteomics
Milk
protein synthesis
secretion
Lipids
Lactation
milk
synthesis
transporters
lactation
lipids
human physiology
Proteins
fat globules
Mastitis
Milk Proteins
Secretory Pathway
milk proteins

Keywords

  • fat globule-membrane
  • mouse mammary-gland
  • proteomic analysis
  • acid transporters
  • epithelial-cells
  • lactation cycle
  • major proteins
  • c2 disease
  • cholesterol
  • expression

Cite this

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title = "Identification of lipid synthesis and secretion proteins in bovine milk",
abstract = "Lactation physiology is a process that is only partly understood. Proteomics techniques have shown to be useful to help advance the knowledge on lactation physiology in human and rodent species but have not been used as major tools for dairy cows, except for mastitis. In this paper, advanced non-targeted proteomics techniques (Filter aided sample preparation and NanoLC-Orbitrap-MS/MS) were applied to study the milk fat globule membrane and milk serum fraction, resulting in the identification of 246 proteins. Of these, 23 transporters and enzymes were related to lipid synthesis and secretion in mammary gland and their functions are discussed in detail. The identification of these intracellular transporters and enzymes in milk provides a possibility of using milk itself to study lipid synthesis and secretion pathways. This full-scale scan of milk proteins by using non-targeted proteomic analysis helps to reveal the important proteins involved in lipid synthesis and secretion for further examination in targeted studies.",
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Identification of lipid synthesis and secretion proteins in bovine milk. / Lu, J.; van Hooijdonk, A.C.M.; Boeren, S.; Vervoort, J.; Hettinga, K.A.

In: Journal of Dairy Research, Vol. 81, No. 1, 2014, p. 65-72.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Identification of lipid synthesis and secretion proteins in bovine milk

AU - Lu, J.

AU - van Hooijdonk, A.C.M.

AU - Boeren, S.

AU - Vervoort, J.

AU - Hettinga, K.A.

PY - 2014

Y1 - 2014

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AB - Lactation physiology is a process that is only partly understood. Proteomics techniques have shown to be useful to help advance the knowledge on lactation physiology in human and rodent species but have not been used as major tools for dairy cows, except for mastitis. In this paper, advanced non-targeted proteomics techniques (Filter aided sample preparation and NanoLC-Orbitrap-MS/MS) were applied to study the milk fat globule membrane and milk serum fraction, resulting in the identification of 246 proteins. Of these, 23 transporters and enzymes were related to lipid synthesis and secretion in mammary gland and their functions are discussed in detail. The identification of these intracellular transporters and enzymes in milk provides a possibility of using milk itself to study lipid synthesis and secretion pathways. This full-scale scan of milk proteins by using non-targeted proteomic analysis helps to reveal the important proteins involved in lipid synthesis and secretion for further examination in targeted studies.

KW - fat globule-membrane

KW - mouse mammary-gland

KW - proteomic analysis

KW - acid transporters

KW - epithelial-cells

KW - lactation cycle

KW - major proteins

KW - c2 disease

KW - cholesterol

KW - expression

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EP - 72

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