Identification of in planta protein–protein interactions using IP-MS

Suraj Jamge, Gerco Angenent, Marian Bemer*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

3 Citations (Scopus)

Abstract

Gene regulation by transcription factors involves complex protein interaction networks, which include chromatin remodeling and modifying proteins as an integral part. Decoding these protein interactions is crucial for our understanding of chromatin-mediated gene regulation. Here, we describe a method for the immunoprecipitation of in planta nuclear protein complexes followed by mass spectrometry (IP-MS) to identify interactions between transcription factors and chromatin remodelers/modifiers in plants. In addition to a step-by-step bench protocol for immunoprecipitation and subsequent mass spectrometry, we provide guidelines and pointers on necessary controls and data analysis approaches.
Original languageEnglish
Title of host publicationPlant Chromatin Dynamics
Subtitle of host publicationMethods and Protocols
EditorsM. Bemer, C. Baroux
PublisherHumana Press Inc.
Pages315-329
ISBN (Electronic)9781493973187
ISBN (Print)9781493973170
DOIs
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1675
ISSN (Print)1064-3745

Keywords

  • Arabidopsis
  • Chromatin remodelers
  • Immunoprecipitation
  • Label free quantification
  • Mass spectrometry
  • Protein–protein interactions

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