Identification of HR-inducing cDNAs from plant pathogens via a Gateway-compatible binary Potato Virus X-expression vector

H.P. van Esse

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Identification of pathogen effectors that elicit a hypersensitive response (HR) in resistant plant hosts is essential to study disease resistance. In this method, it is described how to generate a cDNA library, how to transfer the library into a binary PVX-expression vector, and finally how to set up a high-throughput screen for HR-inducing cDNAs from plant pathogens.
Original languageEnglish
Title of host publicationPlant Fungal Pathogens: Methods and Protocols
EditorsM.D. Bolton, B.P.H.J. Thomma
Place of PublicationNew York, USA
Pages97-105
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
PublisherHumana Press, c/o Springer Science+Business Media
Number835

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  • Cite this

    van Esse, H. P. (2012). Identification of HR-inducing cDNAs from plant pathogens via a Gateway-compatible binary Potato Virus X-expression vector. In M. D. Bolton, & B. P. H. J. Thomma (Eds.), Plant Fungal Pathogens: Methods and Protocols (pp. 97-105). (Methods in Molecular Biology; No. 835).. https://doi.org/10.1007/978-1-61779-501-5_6