Identification of pathogen effectors that elicit a hypersensitive response (HR) in resistant plant hosts is essential to study disease resistance. In this method, it is described how to generate a cDNA library, how to transfer the library into a binary PVX-expression vector, and finally how to set up a high-throughput screen for HR-inducing cDNAs from plant pathogens.
|Title of host publication||Plant Fungal Pathogens: Methods and Protocols|
|Editors||M.D. Bolton, B.P.H.J. Thomma|
|Place of Publication||New York, USA|
|Publication status||Published - 2012|
|Name||Methods in Molecular Biology|
|Publisher||Humana Press, c/o Springer Science+Business Media|
van Esse, H. P. (2012). Identification of HR-inducing cDNAs from plant pathogens via a Gateway-compatible binary Potato Virus X-expression vector. In M. D. Bolton, & B. P. H. J. Thomma (Eds.), Plant Fungal Pathogens: Methods and Protocols (pp. 97-105). (Methods in Molecular Biology; No. 835).. https://doi.org/10.1007/978-1-61779-501-5_6