Identification of Casuarina-Frankia strains by use of polymerase chain reaction (PCR) with arbitrary primers

Anita Sellstedt; Bart Wullings; Ulrika Nyström; Petter Gustafsson

doi:10.1111/j.1574-6968.1992.tb05031.x

Identification of Casuarina-Frankia strains by use of polymerase chain reaction (PCR) with arbitrary primers

Anita Sellstedt^*, Bart Wullings, Ulrika Nyström, Petter Gustafsson

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

29 Citations (Scopus)

Abstract

Free-living N₂-fixing Frankia strains isolated from Casuarina sp. were investigated for genomic polymorphism. We used six 10-mer oligonucleotides as single arbitrary primers (AP) for the polymerase chain reaction (PCR) in order to amplify random DNA fragments in the genome of free-living Frankia strains. Agarose-gels of the amplified genomic DNA revealed that two of the six arbitrary primers showed polymorphism in the eight different Frankia genomes. Analysis of the AP-PCR products showed 9 polymorphic bands ranging from 4.1-0.60 kb. We conclude that single arbitrary primers can be used to amplify genomic DNA, and that polymorphism can be detected between the amplification products of the different Frankia genomes.

Original language	English
Pages (from-to)	1-5
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	93
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6968.1992.tb05031.x
Publication status	Published - May 1992
Externally published	Yes

Keywords

Casuarinaceae
Frankia
Identification
Polymerase chain reaction
Polymorphism

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title = "Identification of Casuarina-Frankia strains by use of polymerase chain reaction (PCR) with arbitrary primers",

abstract = "Free-living N2-fixing Frankia strains isolated from Casuarina sp. were investigated for genomic polymorphism. We used six 10-mer oligonucleotides as single arbitrary primers (AP) for the polymerase chain reaction (PCR) in order to amplify random DNA fragments in the genome of free-living Frankia strains. Agarose-gels of the amplified genomic DNA revealed that two of the six arbitrary primers showed polymorphism in the eight different Frankia genomes. Analysis of the AP-PCR products showed 9 polymorphic bands ranging from 4.1-0.60 kb. We conclude that single arbitrary primers can be used to amplify genomic DNA, and that polymorphism can be detected between the amplification products of the different Frankia genomes.",

keywords = "Casuarinaceae, Frankia, Identification, Polymerase chain reaction, Polymorphism",

author = "Anita Sellstedt and Bart Wullings and Ulrika Nystr{\"o}m and Petter Gustafsson",

year = "1992",

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doi = "10.1111/j.1574-6968.1992.tb05031.x",

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T1 - Identification of Casuarina-Frankia strains by use of polymerase chain reaction (PCR) with arbitrary primers

AU - Sellstedt, Anita

AU - Wullings, Bart

AU - Nyström, Ulrika

AU - Gustafsson, Petter

PY - 1992/5

Y1 - 1992/5

N2 - Free-living N2-fixing Frankia strains isolated from Casuarina sp. were investigated for genomic polymorphism. We used six 10-mer oligonucleotides as single arbitrary primers (AP) for the polymerase chain reaction (PCR) in order to amplify random DNA fragments in the genome of free-living Frankia strains. Agarose-gels of the amplified genomic DNA revealed that two of the six arbitrary primers showed polymorphism in the eight different Frankia genomes. Analysis of the AP-PCR products showed 9 polymorphic bands ranging from 4.1-0.60 kb. We conclude that single arbitrary primers can be used to amplify genomic DNA, and that polymorphism can be detected between the amplification products of the different Frankia genomes.

AB - Free-living N2-fixing Frankia strains isolated from Casuarina sp. were investigated for genomic polymorphism. We used six 10-mer oligonucleotides as single arbitrary primers (AP) for the polymerase chain reaction (PCR) in order to amplify random DNA fragments in the genome of free-living Frankia strains. Agarose-gels of the amplified genomic DNA revealed that two of the six arbitrary primers showed polymorphism in the eight different Frankia genomes. Analysis of the AP-PCR products showed 9 polymorphic bands ranging from 4.1-0.60 kb. We conclude that single arbitrary primers can be used to amplify genomic DNA, and that polymorphism can be detected between the amplification products of the different Frankia genomes.

KW - Casuarinaceae

KW - Frankia

KW - Identification

KW - Polymerase chain reaction

KW - Polymorphism

U2 - 10.1111/j.1574-6968.1992.tb05031.x

DO - 10.1111/j.1574-6968.1992.tb05031.x

M3 - Article

C2 - 1612411

AN - SCOPUS:0026504992

SN - 0378-1097

VL - 93

SP - 1

EP - 5

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1

ER -

Identification of Casuarina-Frankia strains by use of polymerase chain reaction (PCR) with arbitrary primers

Abstract

Keywords

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