Identification of Casuarina-Frankia strains by use of polymerase chain reaction (PCR) with arbitrary primers

Anita Sellstedt*, Bart Wullings, Ulrika Nyström, Petter Gustafsson

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

29 Citations (Scopus)

Abstract

Free-living N2-fixing Frankia strains isolated from Casuarina sp. were investigated for genomic polymorphism. We used six 10-mer oligonucleotides as single arbitrary primers (AP) for the polymerase chain reaction (PCR) in order to amplify random DNA fragments in the genome of free-living Frankia strains. Agarose-gels of the amplified genomic DNA revealed that two of the six arbitrary primers showed polymorphism in the eight different Frankia genomes. Analysis of the AP-PCR products showed 9 polymorphic bands ranging from 4.1-0.60 kb. We conclude that single arbitrary primers can be used to amplify genomic DNA, and that polymorphism can be detected between the amplification products of the different Frankia genomes.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalFEMS Microbiology Letters
Volume93
Issue number1
DOIs
Publication statusPublished - May 1992
Externally publishedYes

Keywords

  • Casuarinaceae
  • Frankia
  • Identification
  • Polymerase chain reaction
  • Polymorphism

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