Identification of breeding signatures in grapevine hybrids, donors of resistances against downy and powdery mildew

S. Vezzulli, E. Peressotti, E. Banchi, C. Dolzani, S. Micheli, M. Stefanini, F. Salamini, R. Velasco, S. Riaz, M.A. Walker, B.I. Reisch, W.E. Van De Weg, M.C. Bink

Research output: Chapter in Book/Report/Conference proceedingConference paper

1 Citation (Scopus)

Abstract

Grapevine (Vitis vinifera L.) is one of the most valuable crops in the world, but has been often plagued by encountering new parasites that still represent a major constraint, such as downy (DM) and powdery (PM) mildew. Nowadays, growers are still obliged to recur to a massive use of pesticides to prevent serious yield loss. In consequence, the research applied to breeding for resistance and the deployment of new resistant grapevine varieties are strongly encouraged as a step towards sustainable agriculture. In this study, we focus on the genetic characterization of numerous Vitis hybrids, donors of resistance to DM and/or PM, in order to identify selection signatures. In particular, 86 hybrids of European origin and of 100 American hybrids, along with 30 related V. vinifera varieties, have been genotyped at 70 SSR loci. Based on the SSR profiles, the historical pedigree information has been checked and, following the trueness-to-type validation for most of the studied hybrids, the Identity By Descent (IBD) analysis has been performed, tracing the allelic flow through the successive generations. Here we report preliminary results about the identification of non-vinifera genomic intervals retained into the vinifera background. Subsequently, the genotyping analysis will be extended to the other 12 chromosomes (120 SSRs) and possibly to new crucial genotypes. The final result of this study will be the release of molecular markers valuable for grapevine Marker-Assisted Breeding (MAB) approach, upon their validation in ad hoc segregating populations.

Original languageEnglish
Title of host publicationActa Horticulturae
PublisherInternational Society for Horticultural Science
Pages145-148
Volume1100
ISBN (Print)9789462610989
DOIs
Publication statusPublished - 2015

Publication series

NameActa Horticulturae
Volume1100
ISSN (Print)0567-7572

Fingerprint

downy mildew
powdery mildew
breeding
Vitis vinifera
Vitis
sustainable agriculture
pedigree
genotyping
growers
pesticides
chromosomes
genomics
parasites
loci
genetic markers
genotype
crops

Keywords

  • Molecular breeding
  • Oidium tuckeri
  • Plasmopara viticola
  • Resistance gene
  • Vitis

Cite this

Vezzulli, S., Peressotti, E., Banchi, E., Dolzani, C., Micheli, S., Stefanini, M., ... Bink, M. C. (2015). Identification of breeding signatures in grapevine hybrids, donors of resistances against downy and powdery mildew. In Acta Horticulturae (Vol. 1100, pp. 145-148). (Acta Horticulturae; Vol. 1100). International Society for Horticultural Science. https://doi.org/10.17660/ActaHortic.2015.1100.22
Vezzulli, S. ; Peressotti, E. ; Banchi, E. ; Dolzani, C. ; Micheli, S. ; Stefanini, M. ; Salamini, F. ; Velasco, R. ; Riaz, S. ; Walker, M.A. ; Reisch, B.I. ; Van De Weg, W.E. ; Bink, M.C. / Identification of breeding signatures in grapevine hybrids, donors of resistances against downy and powdery mildew. Acta Horticulturae. Vol. 1100 International Society for Horticultural Science, 2015. pp. 145-148 (Acta Horticulturae).
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title = "Identification of breeding signatures in grapevine hybrids, donors of resistances against downy and powdery mildew",
abstract = "Grapevine (Vitis vinifera L.) is one of the most valuable crops in the world, but has been often plagued by encountering new parasites that still represent a major constraint, such as downy (DM) and powdery (PM) mildew. Nowadays, growers are still obliged to recur to a massive use of pesticides to prevent serious yield loss. In consequence, the research applied to breeding for resistance and the deployment of new resistant grapevine varieties are strongly encouraged as a step towards sustainable agriculture. In this study, we focus on the genetic characterization of numerous Vitis hybrids, donors of resistance to DM and/or PM, in order to identify selection signatures. In particular, 86 hybrids of European origin and of 100 American hybrids, along with 30 related V. vinifera varieties, have been genotyped at 70 SSR loci. Based on the SSR profiles, the historical pedigree information has been checked and, following the trueness-to-type validation for most of the studied hybrids, the Identity By Descent (IBD) analysis has been performed, tracing the allelic flow through the successive generations. Here we report preliminary results about the identification of non-vinifera genomic intervals retained into the vinifera background. Subsequently, the genotyping analysis will be extended to the other 12 chromosomes (120 SSRs) and possibly to new crucial genotypes. The final result of this study will be the release of molecular markers valuable for grapevine Marker-Assisted Breeding (MAB) approach, upon their validation in ad hoc segregating populations.",
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author = "S. Vezzulli and E. Peressotti and E. Banchi and C. Dolzani and S. Micheli and M. Stefanini and F. Salamini and R. Velasco and S. Riaz and M.A. Walker and B.I. Reisch and {Van De Weg}, W.E. and M.C. Bink",
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Vezzulli, S, Peressotti, E, Banchi, E, Dolzani, C, Micheli, S, Stefanini, M, Salamini, F, Velasco, R, Riaz, S, Walker, MA, Reisch, BI, Van De Weg, WE & Bink, MC 2015, Identification of breeding signatures in grapevine hybrids, donors of resistances against downy and powdery mildew. in Acta Horticulturae. vol. 1100, Acta Horticulturae, vol. 1100, International Society for Horticultural Science, pp. 145-148. https://doi.org/10.17660/ActaHortic.2015.1100.22

Identification of breeding signatures in grapevine hybrids, donors of resistances against downy and powdery mildew. / Vezzulli, S.; Peressotti, E.; Banchi, E.; Dolzani, C.; Micheli, S.; Stefanini, M.; Salamini, F.; Velasco, R.; Riaz, S.; Walker, M.A.; Reisch, B.I.; Van De Weg, W.E.; Bink, M.C.

Acta Horticulturae. Vol. 1100 International Society for Horticultural Science, 2015. p. 145-148 (Acta Horticulturae; Vol. 1100).

Research output: Chapter in Book/Report/Conference proceedingConference paper

TY - GEN

T1 - Identification of breeding signatures in grapevine hybrids, donors of resistances against downy and powdery mildew

AU - Vezzulli, S.

AU - Peressotti, E.

AU - Banchi, E.

AU - Dolzani, C.

AU - Micheli, S.

AU - Stefanini, M.

AU - Salamini, F.

AU - Velasco, R.

AU - Riaz, S.

AU - Walker, M.A.

AU - Reisch, B.I.

AU - Van De Weg, W.E.

AU - Bink, M.C.

PY - 2015

Y1 - 2015

N2 - Grapevine (Vitis vinifera L.) is one of the most valuable crops in the world, but has been often plagued by encountering new parasites that still represent a major constraint, such as downy (DM) and powdery (PM) mildew. Nowadays, growers are still obliged to recur to a massive use of pesticides to prevent serious yield loss. In consequence, the research applied to breeding for resistance and the deployment of new resistant grapevine varieties are strongly encouraged as a step towards sustainable agriculture. In this study, we focus on the genetic characterization of numerous Vitis hybrids, donors of resistance to DM and/or PM, in order to identify selection signatures. In particular, 86 hybrids of European origin and of 100 American hybrids, along with 30 related V. vinifera varieties, have been genotyped at 70 SSR loci. Based on the SSR profiles, the historical pedigree information has been checked and, following the trueness-to-type validation for most of the studied hybrids, the Identity By Descent (IBD) analysis has been performed, tracing the allelic flow through the successive generations. Here we report preliminary results about the identification of non-vinifera genomic intervals retained into the vinifera background. Subsequently, the genotyping analysis will be extended to the other 12 chromosomes (120 SSRs) and possibly to new crucial genotypes. The final result of this study will be the release of molecular markers valuable for grapevine Marker-Assisted Breeding (MAB) approach, upon their validation in ad hoc segregating populations.

AB - Grapevine (Vitis vinifera L.) is one of the most valuable crops in the world, but has been often plagued by encountering new parasites that still represent a major constraint, such as downy (DM) and powdery (PM) mildew. Nowadays, growers are still obliged to recur to a massive use of pesticides to prevent serious yield loss. In consequence, the research applied to breeding for resistance and the deployment of new resistant grapevine varieties are strongly encouraged as a step towards sustainable agriculture. In this study, we focus on the genetic characterization of numerous Vitis hybrids, donors of resistance to DM and/or PM, in order to identify selection signatures. In particular, 86 hybrids of European origin and of 100 American hybrids, along with 30 related V. vinifera varieties, have been genotyped at 70 SSR loci. Based on the SSR profiles, the historical pedigree information has been checked and, following the trueness-to-type validation for most of the studied hybrids, the Identity By Descent (IBD) analysis has been performed, tracing the allelic flow through the successive generations. Here we report preliminary results about the identification of non-vinifera genomic intervals retained into the vinifera background. Subsequently, the genotyping analysis will be extended to the other 12 chromosomes (120 SSRs) and possibly to new crucial genotypes. The final result of this study will be the release of molecular markers valuable for grapevine Marker-Assisted Breeding (MAB) approach, upon their validation in ad hoc segregating populations.

KW - Molecular breeding

KW - Oidium tuckeri

KW - Plasmopara viticola

KW - Resistance gene

KW - Vitis

U2 - 10.17660/ActaHortic.2015.1100.22

DO - 10.17660/ActaHortic.2015.1100.22

M3 - Conference paper

SN - 9789462610989

VL - 1100

T3 - Acta Horticulturae

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BT - Acta Horticulturae

PB - International Society for Horticultural Science

ER -

Vezzulli S, Peressotti E, Banchi E, Dolzani C, Micheli S, Stefanini M et al. Identification of breeding signatures in grapevine hybrids, donors of resistances against downy and powdery mildew. In Acta Horticulturae. Vol. 1100. International Society for Horticultural Science. 2015. p. 145-148. (Acta Horticulturae). https://doi.org/10.17660/ActaHortic.2015.1100.22