Identification of Bilophila wadsworthia by specific PCR which targets the taurine:pyruvate aminotransferase

H. Laue, T.H.M. Smits, U.K. Schumacher, M.C. Claros, R. Hartemink, A.M. Cook

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

The bile-resistant, strictly anaerobic bacterium Bilophila wadsworthia is found in human faecal flora, in human infections and in environmental samples. A specific PCR primer set for the gene encoding the first metabolic enzyme in the degradative pathway for taurine in B. wadsworthia, taurine:pyruvate aminotransferase (tpa), was developed and tested. In addition, enrichment cultures were started from faecal samples of primates and felines and shown to contain B. wadsworthia. These were subcultured on agar media and then identified by PCR fingerprinting. PCR for tpa was successful in all positive enrichment cultures and showed no amplification signal in a variety of other bacterial species. Therefore, this PCR method could be a promising tool for rapid detection of B. wadsworthia in biological samples
Original languageEnglish
Pages (from-to)74-79
JournalFEMS Microbiology Letters
Volume261
Issue number1
DOIs
Publication statusPublished - 2006

Keywords

  • sp-nov
  • transcription
  • bacteria
  • quantification
  • dissimilation
  • purification
  • appendicitis
  • respiration
  • reduction
  • specimens

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