Identification of an unknown Beta-agonist in feed by liquid chromatography/bioassay/quadrupole time-of-flight tandem mass spectrometry with accurate mass measurement

M.W.F. Nielen, C.T. Elliott, S.A. Boyd, D. Courtheyn, M.L. Essers, H.H. Hooijerink, E.O. van Bennekom

Research output: Contribution to journalArticleAcademicpeer-review

47 Citations (Scopus)

Abstract

A new approach to the search for residues of unknown growth promoting agents such as anabolic steroids and -agonists in feed is presented. Following primary extraction and clean-up, samples are separated using gradient liquid chromatography (LC). The effluent is split towards two identical 96-well fraction collectors and an optional electrospray quadrupole time-of-flight mass spectrometry (QTOFMS) system for accurate mass measurement. One 96-well plate is used for a bioassay (enzyme-immuno assay, receptor assay) and will detect the bioactivity and position of the relevant peak in the chromatogram. The positive well in the second 96-well plate is used for identification by LC/QTOFMS/MS. The value of this LC/bioassay/QTOFMS/MS methodology is highlighted by the finding and structure elucidation of a new -agonist in a feed extract.
Original languageEnglish
Pages (from-to)1633-1641
JournalRapid Communications in Mass Spectrometry
Volume17
Issue number14
DOIs
Publication statusPublished - 2003

Keywords

  • urine
  • chromatography
  • online
  • clenbuterol
  • extraction

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