Identification of a large-effect QTL associated with kernel discoloration in barley

Cynara C.T. Romero, Anton Vels, Rients E. Niks*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Three barley mapping populations sharing the parental line SusPtrit suffered from an unknown condition affecting grain quality and germination. Grains showed dark blotches or even whole-grain discoloration and shrivelling. Symptoms were similar to kernel discoloration as reported in wheat and barley. Some covered-seed lines affected by this quality condition produced grains that were either not covered at all or in which partial hull loss was observed, a phenotype that resembles that of grain skinning. No fungal or bacterial agent was identified as causal organism, and we demonstrate that the poor quality phenotype is not transmitted to the next generation – therefore unlikely due to a seed borne pathogen. A major effect QTL on chromosome 6H was mapped independently in all three populations, with the poor quality allele contributed by SusPtrit. A second, minor effect QTL, was mapped on chromosome 2H in the SusPtrit x Golden Promise population, with Golden Promise as the parent donor of the allele conferring poor quality. The grain quality disorder is not linked to the nud gene on chromosome 7H for the naked/covered seed trait.

Original languageEnglish
Pages (from-to)62-70
JournalJournal of Cereal Science
Volume84
DOIs
Publication statusPublished - Nov 2018

Fingerprint

Discoloration
Hordeum
discoloration
quantitative trait loci
Seeds
Chromosomes
barley
seeds
chromosomes
Alleles
Population
Phenotype
Seed
skinning
Germination
alleles
phenotype
Triticum
whole grain foods
hulls

Keywords

  • Black point
  • Grain skinning
  • Kernel discoloration
  • QTL mapping
  • Seed quality

Cite this

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title = "Identification of a large-effect QTL associated with kernel discoloration in barley",
abstract = "Three barley mapping populations sharing the parental line SusPtrit suffered from an unknown condition affecting grain quality and germination. Grains showed dark blotches or even whole-grain discoloration and shrivelling. Symptoms were similar to kernel discoloration as reported in wheat and barley. Some covered-seed lines affected by this quality condition produced grains that were either not covered at all or in which partial hull loss was observed, a phenotype that resembles that of grain skinning. No fungal or bacterial agent was identified as causal organism, and we demonstrate that the poor quality phenotype is not transmitted to the next generation – therefore unlikely due to a seed borne pathogen. A major effect QTL on chromosome 6H was mapped independently in all three populations, with the poor quality allele contributed by SusPtrit. A second, minor effect QTL, was mapped on chromosome 2H in the SusPtrit x Golden Promise population, with Golden Promise as the parent donor of the allele conferring poor quality. The grain quality disorder is not linked to the nud gene on chromosome 7H for the naked/covered seed trait.",
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author = "Romero, {Cynara C.T.} and Anton Vels and Niks, {Rients E.}",
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language = "English",
volume = "84",
pages = "62--70",
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Identification of a large-effect QTL associated with kernel discoloration in barley. / Romero, Cynara C.T.; Vels, Anton; Niks, Rients E.

In: Journal of Cereal Science, Vol. 84, 11.2018, p. 62-70.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Identification of a large-effect QTL associated with kernel discoloration in barley

AU - Romero, Cynara C.T.

AU - Vels, Anton

AU - Niks, Rients E.

PY - 2018/11

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N2 - Three barley mapping populations sharing the parental line SusPtrit suffered from an unknown condition affecting grain quality and germination. Grains showed dark blotches or even whole-grain discoloration and shrivelling. Symptoms were similar to kernel discoloration as reported in wheat and barley. Some covered-seed lines affected by this quality condition produced grains that were either not covered at all or in which partial hull loss was observed, a phenotype that resembles that of grain skinning. No fungal or bacterial agent was identified as causal organism, and we demonstrate that the poor quality phenotype is not transmitted to the next generation – therefore unlikely due to a seed borne pathogen. A major effect QTL on chromosome 6H was mapped independently in all three populations, with the poor quality allele contributed by SusPtrit. A second, minor effect QTL, was mapped on chromosome 2H in the SusPtrit x Golden Promise population, with Golden Promise as the parent donor of the allele conferring poor quality. The grain quality disorder is not linked to the nud gene on chromosome 7H for the naked/covered seed trait.

AB - Three barley mapping populations sharing the parental line SusPtrit suffered from an unknown condition affecting grain quality and germination. Grains showed dark blotches or even whole-grain discoloration and shrivelling. Symptoms were similar to kernel discoloration as reported in wheat and barley. Some covered-seed lines affected by this quality condition produced grains that were either not covered at all or in which partial hull loss was observed, a phenotype that resembles that of grain skinning. No fungal or bacterial agent was identified as causal organism, and we demonstrate that the poor quality phenotype is not transmitted to the next generation – therefore unlikely due to a seed borne pathogen. A major effect QTL on chromosome 6H was mapped independently in all three populations, with the poor quality allele contributed by SusPtrit. A second, minor effect QTL, was mapped on chromosome 2H in the SusPtrit x Golden Promise population, with Golden Promise as the parent donor of the allele conferring poor quality. The grain quality disorder is not linked to the nud gene on chromosome 7H for the naked/covered seed trait.

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KW - Grain skinning

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