Abstract
| Original language | English |
|---|---|
| Publication status | Published - 2014 |
| Event | 50th Anniversary Dutch Society Immunology.nl (NVVI), Kaatsheuvel, NL - Duration: 17 Dec 2014 → 19 Dec 2014 |
Conference
| Conference | 50th Anniversary Dutch Society Immunology.nl (NVVI), Kaatsheuvel, NL |
|---|---|
| Period | 17/12/14 → 19/12/14 |
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Identification and production of recombinant Culicoides obsoletus allergens for diagnosis and immunotherapy of insect bite hypersensitive horses. / van der Meide, N.M.A.; Schurink, A.; Sloet van Oldruitenborgh-Oosterbaan, M.M.; Schaap, P.J.; Savelkoul, H.F.J.; Tijhaar, E.J.
2014. Abstract from 50th Anniversary Dutch Society Immunology.nl (NVVI), Kaatsheuvel, NL, .Research output: Contribution to conference › Abstract › Academic
TY - CONF
T1 - Identification and production of recombinant Culicoides obsoletus allergens for diagnosis and immunotherapy of insect bite hypersensitive horses
AU - van der Meide, N.M.A.
AU - Schurink, A.
AU - Sloet van Oldruitenborgh-Oosterbaan, M.M.
AU - Schaap, P.J.
AU - Savelkoul, H.F.J.
AU - Tijhaar, E.J.
PY - 2014
Y1 - 2014
N2 - Insect Bite Hypersensitivity (IBH) is a common IgE mediated skin allergy in horses, caused by the bites of Culicoides species (midges) that seriously affects the welfare of these horses. Currently no effective treatment and no reliable diagnostic test is available for this allergic condition. We identified midges of the C. obsoletus complex as the dominant Culicoides species feeding on horses in the Netherlands. Its transcriptome was determined and sequence similarity searches with allergens previously described forother midges (C. sonorensis and C. nubeculosus) revealed 7 potential allergens of C. obsoletus , named Cul o 1 – Cul o 7.The sequences encoding these allergens were cloned and expressed as his6-tagged recombinant proteins in E. coli andpurified on Ni2+ sepharose beads. The purified recombinant proteins were able to induce an allergic reaction after injection into the skin of IBH affected horses, but not in healthy control horses. They all showed significant (p
AB - Insect Bite Hypersensitivity (IBH) is a common IgE mediated skin allergy in horses, caused by the bites of Culicoides species (midges) that seriously affects the welfare of these horses. Currently no effective treatment and no reliable diagnostic test is available for this allergic condition. We identified midges of the C. obsoletus complex as the dominant Culicoides species feeding on horses in the Netherlands. Its transcriptome was determined and sequence similarity searches with allergens previously described forother midges (C. sonorensis and C. nubeculosus) revealed 7 potential allergens of C. obsoletus , named Cul o 1 – Cul o 7.The sequences encoding these allergens were cloned and expressed as his6-tagged recombinant proteins in E. coli andpurified on Ni2+ sepharose beads. The purified recombinant proteins were able to induce an allergic reaction after injection into the skin of IBH affected horses, but not in healthy control horses. They all showed significant (p
M3 - Abstract
ER -