Identification and production of recombinant Culicoides obsoletus allergens for diagnosis and immunotherapy of insect bite hypersensitive horses

N.M.A. van der Meide, A. Schurink, M.M. Sloet van Oldruitenborgh-Oosterbaan, P.J. Schaap, H.F.J. Savelkoul, E.J. Tijhaar

Research output: Contribution to conferenceAbstractAcademic

Abstract

Insect Bite Hypersensitivity (IBH) is a common IgE mediated skin allergy in horses, caused by the bites of Culicoides species (midges) that seriously affects the welfare of these horses. Currently no effective treatment and no reliable diagnostic test is available for this allergic condition. We identified midges of the C. obsoletus complex as the dominant Culicoides species feeding on horses in the Netherlands. Its transcriptome was determined and sequence similarity searches with allergens previously described forother midges (C. sonorensis and C. nubeculosus) revealed 7 potential allergens of C. obsoletus , named Cul o 1 – Cul o 7.The sequences encoding these allergens were cloned and expressed as his6-tagged recombinant proteins in E. coli andpurified on Ni2+ sepharose beads. The purified recombinant proteins were able to induce an allergic reaction after injection into the skin of IBH affected horses, but not in healthy control horses. They all showed significant (p
Original languageEnglish
Publication statusPublished - 2014
Event50th Anniversary Dutch Society Immunology.nl (NVVI), Kaatsheuvel, NL -
Duration: 17 Dec 201419 Dec 2014

Conference

Conference50th Anniversary Dutch Society Immunology.nl (NVVI), Kaatsheuvel, NL
Period17/12/1419/12/14

Fingerprint

Culicoides obsoletus
immunotherapy
allergens
hypersensitivity
midges
horses
insects
Culicoides
recombinant proteins
Culicoides sonorensis
transcriptome
diagnostic techniques
agarose
Netherlands
Escherichia coli
injection

Cite this

van der Meide, N. M. A., Schurink, A., Sloet van Oldruitenborgh-Oosterbaan, M. M., Schaap, P. J., Savelkoul, H. F. J., & Tijhaar, E. J. (2014). Identification and production of recombinant Culicoides obsoletus allergens for diagnosis and immunotherapy of insect bite hypersensitive horses. Abstract from 50th Anniversary Dutch Society Immunology.nl (NVVI), Kaatsheuvel, NL, .
van der Meide, N.M.A. ; Schurink, A. ; Sloet van Oldruitenborgh-Oosterbaan, M.M. ; Schaap, P.J. ; Savelkoul, H.F.J. ; Tijhaar, E.J. / Identification and production of recombinant Culicoides obsoletus allergens for diagnosis and immunotherapy of insect bite hypersensitive horses. Abstract from 50th Anniversary Dutch Society Immunology.nl (NVVI), Kaatsheuvel, NL, .
@conference{d785085cab3e4f21901a1cc61d84b16b,
title = "Identification and production of recombinant Culicoides obsoletus allergens for diagnosis and immunotherapy of insect bite hypersensitive horses",
abstract = "Insect Bite Hypersensitivity (IBH) is a common IgE mediated skin allergy in horses, caused by the bites of Culicoides species (midges) that seriously affects the welfare of these horses. Currently no effective treatment and no reliable diagnostic test is available for this allergic condition. We identified midges of the C. obsoletus complex as the dominant Culicoides species feeding on horses in the Netherlands. Its transcriptome was determined and sequence similarity searches with allergens previously described forother midges (C. sonorensis and C. nubeculosus) revealed 7 potential allergens of C. obsoletus , named Cul o 1 – Cul o 7.The sequences encoding these allergens were cloned and expressed as his6-tagged recombinant proteins in E. coli andpurified on Ni2+ sepharose beads. The purified recombinant proteins were able to induce an allergic reaction after injection into the skin of IBH affected horses, but not in healthy control horses. They all showed significant (p",
author = "{van der Meide}, N.M.A. and A. Schurink and {Sloet van Oldruitenborgh-Oosterbaan}, M.M. and P.J. Schaap and H.F.J. Savelkoul and E.J. Tijhaar",
year = "2014",
language = "English",
note = "50th Anniversary Dutch Society Immunology.nl (NVVI), Kaatsheuvel, NL ; Conference date: 17-12-2014 Through 19-12-2014",

}

van der Meide, NMA, Schurink, A, Sloet van Oldruitenborgh-Oosterbaan, MM, Schaap, PJ, Savelkoul, HFJ & Tijhaar, EJ 2014, 'Identification and production of recombinant Culicoides obsoletus allergens for diagnosis and immunotherapy of insect bite hypersensitive horses' 50th Anniversary Dutch Society Immunology.nl (NVVI), Kaatsheuvel, NL, 17/12/14 - 19/12/14, .

Identification and production of recombinant Culicoides obsoletus allergens for diagnosis and immunotherapy of insect bite hypersensitive horses. / van der Meide, N.M.A.; Schurink, A.; Sloet van Oldruitenborgh-Oosterbaan, M.M.; Schaap, P.J.; Savelkoul, H.F.J.; Tijhaar, E.J.

2014. Abstract from 50th Anniversary Dutch Society Immunology.nl (NVVI), Kaatsheuvel, NL, .

Research output: Contribution to conferenceAbstractAcademic

TY - CONF

T1 - Identification and production of recombinant Culicoides obsoletus allergens for diagnosis and immunotherapy of insect bite hypersensitive horses

AU - van der Meide, N.M.A.

AU - Schurink, A.

AU - Sloet van Oldruitenborgh-Oosterbaan, M.M.

AU - Schaap, P.J.

AU - Savelkoul, H.F.J.

AU - Tijhaar, E.J.

PY - 2014

Y1 - 2014

N2 - Insect Bite Hypersensitivity (IBH) is a common IgE mediated skin allergy in horses, caused by the bites of Culicoides species (midges) that seriously affects the welfare of these horses. Currently no effective treatment and no reliable diagnostic test is available for this allergic condition. We identified midges of the C. obsoletus complex as the dominant Culicoides species feeding on horses in the Netherlands. Its transcriptome was determined and sequence similarity searches with allergens previously described forother midges (C. sonorensis and C. nubeculosus) revealed 7 potential allergens of C. obsoletus , named Cul o 1 – Cul o 7.The sequences encoding these allergens were cloned and expressed as his6-tagged recombinant proteins in E. coli andpurified on Ni2+ sepharose beads. The purified recombinant proteins were able to induce an allergic reaction after injection into the skin of IBH affected horses, but not in healthy control horses. They all showed significant (p

AB - Insect Bite Hypersensitivity (IBH) is a common IgE mediated skin allergy in horses, caused by the bites of Culicoides species (midges) that seriously affects the welfare of these horses. Currently no effective treatment and no reliable diagnostic test is available for this allergic condition. We identified midges of the C. obsoletus complex as the dominant Culicoides species feeding on horses in the Netherlands. Its transcriptome was determined and sequence similarity searches with allergens previously described forother midges (C. sonorensis and C. nubeculosus) revealed 7 potential allergens of C. obsoletus , named Cul o 1 – Cul o 7.The sequences encoding these allergens were cloned and expressed as his6-tagged recombinant proteins in E. coli andpurified on Ni2+ sepharose beads. The purified recombinant proteins were able to induce an allergic reaction after injection into the skin of IBH affected horses, but not in healthy control horses. They all showed significant (p

M3 - Abstract

ER -

van der Meide NMA, Schurink A, Sloet van Oldruitenborgh-Oosterbaan MM, Schaap PJ, Savelkoul HFJ, Tijhaar EJ. Identification and production of recombinant Culicoides obsoletus allergens for diagnosis and immunotherapy of insect bite hypersensitive horses. 2014. Abstract from 50th Anniversary Dutch Society Immunology.nl (NVVI), Kaatsheuvel, NL, .