Identification and management of virulence genes in potato cyst nematodes

F.J. Gommers, J. Roosien, A. Schouten, J.M. de Boer, H.A. Overmars, L. Bouwman-Smits, R. Folkertsma, P. van Zandvoort, M. van Gent-Pelzer, A. Schots, R. Janssen, J. Bakker

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

Efficient and accurate diagnostic assays are essential for the design and evaluation of control measures of the potato cyst nematodesGlobodera rostochiensis andG. pallida by means of resistance. The hybridoma technology and the polymerase chain reaction (PCR) offer in potential various possibilities to design such diagnostic tests for routine purposes. We set out to devise a refined advisory system based on biochemical assays by using the following stepwise approach.
Original languageEnglish
Pages (from-to)157-163
JournalNetherlands Journal of Plant Pathology
Volume98
Issue numbersuppl.2
DOIs
Publication statusPublished - 1992

Keywords

  • 2D-gel electrophoresis
  • G. pallida
  • Globodera rostochiensis
  • hybridoma
  • pathotypes
  • PCR
  • RAPD
  • virulence genes

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