Identification and functional characterization of the Arabidopsis Snf1-related protein kinase SnRK2.4 phosphatidic acid-binding domain

Magdalena M. Julkowska, Fionn McLoughlin, Carlos S. Galvan-Ampudia, Johanna M. Rankenberg, Dorota Kawa, Maria Klimecka, Michel A. Haring, Teun Munnik, Edgar E. Kooijman, Christa Testerink

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23 Citations (Scopus)

Abstract

Phosphatidic acid (PA) is an important signalling lipid involved in various stress-induced signalling cascades. Two SnRK2 protein kinases (SnRK2.4 and SnRK2.10), previously identified as PA-binding proteins, are shown here to prefer binding to PA over other anionic phospholipids and to associate with cellular membranes in response to salt stress in Arabidopsis roots. A 42 amino acid sequence was identified as the primary PA-binding domain (PABD) of SnRK2.4. Unlike the full-length SnRK2.4, neither the PABD-YFP fusion protein nor the SnRK2.10 re-localized into punctate structures upon salt stress treatment, showing that additional domains of the SnRK2.4 protein are required for its re-localization during salt stress. Within the PABD, five basic amino acids, conserved in class 1 SnRK2s, were found to be necessary for PA binding. Remarkably, plants overexpressing the PABD, but not a non-PA-binding mutant version, showed a severe reduction in root growth. Together, this study biochemically characterizes the PA-SnRK2.4 interaction and shows that functionality of the SnRK2.4 PABD affects root development.

Original languageEnglish
Pages (from-to)614-624
Number of pages11
JournalPlant, Cell & Environment
Volume38
Issue number3
DOIs
Publication statusPublished - Mar 2015

Keywords

  • phosphatidic acid
  • phospholipid binding
  • root system architecture
  • SnRK2.10

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    Julkowska, M. M., McLoughlin, F., Galvan-Ampudia, C. S., Rankenberg, J. M., Kawa, D., Klimecka, M., Haring, M. A., Munnik, T., Kooijman, E. E., & Testerink, C. (2015). Identification and functional characterization of the Arabidopsis Snf1-related protein kinase SnRK2.4 phosphatidic acid-binding domain. Plant, Cell & Environment, 38(3), 614-624. https://doi.org/10.1111/pce.12421