Identification and functional analysis of two ZIP metal transporters of the hyperaccumulator Thlaspi caerulescens

J. Wu; F.J. Zhao; A. Ghandilyan; B. Logoteta; M.C. Olortegui Guzman; H. Schat; X. Wang; M.G.M. Aarts

doi:10.1007/s11104-009-0151-6

Identification and functional analysis of two ZIP metal transporters of the hyperaccumulator Thlaspi caerulescens

J. Wu, F.J. Zhao, A. Ghandilyan, B. Logoteta, M.C. Olortegui Guzman, H. Schat, X. Wang, M.G.M. Aarts

Research output: Contribution to journal › Article › Academic › peer-review

42 Citations (Scopus)

Abstract

Abstract The heavy metal hyperaccumulator Thlaspi caerulescens expresses several ZIP-like genes at higher levels than their orthologues in non-hyperaccumulator species, but it is not clear why. To elucidate the function of the T. caerulescens orthologues of the Arabidopsis thaliana ZIP5 and ZIP6 genes, full-length cDNAs of TcZNT5-LC and TcZNT6-LC were cloned, their expression was examined and genes were expressed in A. thaliana. Transcript level analysis revealed the constitutively high expression of these two genes in T. caerulescens compared to AtZIP5 and AtZIP6 genes and differential expression of both genes when comparing two accessions of T. caerulescens with different metal accumulation properties. Expression of TcZNT5-LC in A. thaliana did not modify Cd or Zn tolerance, but mildly affected the root and shoot Zn concentrations towards a hyperaccumulator shoot to root concentration ratio. A. thaliana zip5 knock-out mutants showed increased tolerance to Cd and decreased seed mineral concentrations. Expression of TcZNT6-LC enhanced the Cd sensitivity of A. thaliana, but no phenotype was observed for the zip6 mutant. In conclusion, the changes in expression of TcZNT5-LC and TcZNT6-LC upon changes in Zn or Cd exposure indicate both genes act in metal homeostasis, but their CaMV 35S-mediated expression in A. thaliana does not create T. caerulescens hyperaccumulator phenotypes

Original language	English
Pages (from-to)	79-95
Journal	Plant and Soil
Volume	325
Issue number	1-2
DOIs	https://doi.org/10.1007/s11104-009-0151-6
Publication status	Published - 2009

Keywords

arabidopsis-thaliana
zinc transporter
expression differences
genes
iron
homeostasis
irt1
sequence
family
accumulation

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10.1007/s11104-009-0151-6

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title = "Identification and functional analysis of two ZIP metal transporters of the hyperaccumulator Thlaspi caerulescens",

abstract = "Abstract The heavy metal hyperaccumulator Thlaspi caerulescens expresses several ZIP-like genes at higher levels than their orthologues in non-hyperaccumulator species, but it is not clear why. To elucidate the function of the T. caerulescens orthologues of the Arabidopsis thaliana ZIP5 and ZIP6 genes, full-length cDNAs of TcZNT5-LC and TcZNT6-LC were cloned, their expression was examined and genes were expressed in A. thaliana. Transcript level analysis revealed the constitutively high expression of these two genes in T. caerulescens compared to AtZIP5 and AtZIP6 genes and differential expression of both genes when comparing two accessions of T. caerulescens with different metal accumulation properties. Expression of TcZNT5-LC in A. thaliana did not modify Cd or Zn tolerance, but mildly affected the root and shoot Zn concentrations towards a hyperaccumulator shoot to root concentration ratio. A. thaliana zip5 knock-out mutants showed increased tolerance to Cd and decreased seed mineral concentrations. Expression of TcZNT6-LC enhanced the Cd sensitivity of A. thaliana, but no phenotype was observed for the zip6 mutant. In conclusion, the changes in expression of TcZNT5-LC and TcZNT6-LC upon changes in Zn or Cd exposure indicate both genes act in metal homeostasis, but their CaMV 35S-mediated expression in A. thaliana does not create T. caerulescens hyperaccumulator phenotypes",

keywords = "arabidopsis-thaliana, zinc transporter, expression differences, genes, iron, homeostasis, irt1, sequence, family, accumulation",

author = "J. Wu and F.J. Zhao and A. Ghandilyan and B. Logoteta and {Olortegui Guzman}, M.C. and H. Schat and X. Wang and M.G.M. Aarts",

year = "2009",

doi = "10.1007/s11104-009-0151-6",

language = "English",

volume = "325",

pages = "79--95",

journal = "Plant and Soil",

issn = "0032-079X",

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TY - JOUR

T1 - Identification and functional analysis of two ZIP metal transporters of the hyperaccumulator Thlaspi caerulescens

AU - Wu, J.

AU - Zhao, F.J.

AU - Ghandilyan, A.

AU - Logoteta, B.

AU - Olortegui Guzman, M.C.

AU - Schat, H.

AU - Wang, X.

AU - Aarts, M.G.M.

PY - 2009

Y1 - 2009

N2 - Abstract The heavy metal hyperaccumulator Thlaspi caerulescens expresses several ZIP-like genes at higher levels than their orthologues in non-hyperaccumulator species, but it is not clear why. To elucidate the function of the T. caerulescens orthologues of the Arabidopsis thaliana ZIP5 and ZIP6 genes, full-length cDNAs of TcZNT5-LC and TcZNT6-LC were cloned, their expression was examined and genes were expressed in A. thaliana. Transcript level analysis revealed the constitutively high expression of these two genes in T. caerulescens compared to AtZIP5 and AtZIP6 genes and differential expression of both genes when comparing two accessions of T. caerulescens with different metal accumulation properties. Expression of TcZNT5-LC in A. thaliana did not modify Cd or Zn tolerance, but mildly affected the root and shoot Zn concentrations towards a hyperaccumulator shoot to root concentration ratio. A. thaliana zip5 knock-out mutants showed increased tolerance to Cd and decreased seed mineral concentrations. Expression of TcZNT6-LC enhanced the Cd sensitivity of A. thaliana, but no phenotype was observed for the zip6 mutant. In conclusion, the changes in expression of TcZNT5-LC and TcZNT6-LC upon changes in Zn or Cd exposure indicate both genes act in metal homeostasis, but their CaMV 35S-mediated expression in A. thaliana does not create T. caerulescens hyperaccumulator phenotypes

AB - Abstract The heavy metal hyperaccumulator Thlaspi caerulescens expresses several ZIP-like genes at higher levels than their orthologues in non-hyperaccumulator species, but it is not clear why. To elucidate the function of the T. caerulescens orthologues of the Arabidopsis thaliana ZIP5 and ZIP6 genes, full-length cDNAs of TcZNT5-LC and TcZNT6-LC were cloned, their expression was examined and genes were expressed in A. thaliana. Transcript level analysis revealed the constitutively high expression of these two genes in T. caerulescens compared to AtZIP5 and AtZIP6 genes and differential expression of both genes when comparing two accessions of T. caerulescens with different metal accumulation properties. Expression of TcZNT5-LC in A. thaliana did not modify Cd or Zn tolerance, but mildly affected the root and shoot Zn concentrations towards a hyperaccumulator shoot to root concentration ratio. A. thaliana zip5 knock-out mutants showed increased tolerance to Cd and decreased seed mineral concentrations. Expression of TcZNT6-LC enhanced the Cd sensitivity of A. thaliana, but no phenotype was observed for the zip6 mutant. In conclusion, the changes in expression of TcZNT5-LC and TcZNT6-LC upon changes in Zn or Cd exposure indicate both genes act in metal homeostasis, but their CaMV 35S-mediated expression in A. thaliana does not create T. caerulescens hyperaccumulator phenotypes

KW - arabidopsis-thaliana

KW - zinc transporter

KW - expression differences

KW - genes

KW - iron

KW - homeostasis

KW - irt1

KW - sequence

KW - family

KW - accumulation

U2 - 10.1007/s11104-009-0151-6

DO - 10.1007/s11104-009-0151-6

M3 - Article

SN - 0032-079X

VL - 325

SP - 79

EP - 95

JO - Plant and Soil

JF - Plant and Soil

IS - 1-2

ER -

Identification and functional analysis of two ZIP metal transporters of the hyperaccumulator Thlaspi caerulescens

Abstract

Keywords

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