Identification and full characterisation of two novel crustacean infecting members of the family nudiviridae provides support for two subfamilies

Kelly S. Bateman*, Rose Kerr, Grant D. Stentiford, Tim P. Bean, Chantelle Hooper, Benigna Van Eynde, Daan Delbare, Jamie Bojko, Olivier Christiaens, Clauvis N.T. Taning, Guy Smagghe, Monique M. van Oers, Ronny van Aerle

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Multiple enveloped viruses with rod-shaped nucleocapsids have been described, infecting the epithelial cell nuclei within the hepatopancreas tubules of crustaceans. These bacilliform viruses share the ultrastructural characteristics of nudiviruses, a specific clade of viruses infecting arthropods. Using histology, electron microscopy and high throughput sequencing, we characterise two further bacilliform viruses from aquatic hosts, the brown shrimp (Crangon crangon) and the European shore crab (Carcinus maenas). We assembled the full double stranded, circular DNA genome sequences of these viruses (~113 and 132 kbp, respectively). Comparative genomics and phylogenetic analyses confirm that both belong within the family Nudiviridae but in separate clades representing nudiviruses found in freshwater and marine environments. We show that the three thymidine kinase (tk) genes present in all sequenced nudivirus genomes, thus far, were absent in the Crangon crangon nudivirus, suggesting there are twenty-eight core genes shared by all nudi-viruses. Furthermore, the phylogenetic data no longer support the subdivision of the family Nudi-viridae into four genera (Alphanudivirus to Deltanudivirus), as recently adopted by the International Committee on Taxonomy of Viruses (ICTV), but rather shows two main branches of the family that are further subdivided. Our data support a recent proposal to create two subfamilies within the family Nudiviridae, each subdivided into several genera.

Original languageEnglish
Article number1694
JournalViruses
Volume13
Issue number9
DOIs
Publication statusPublished - 26 Aug 2021

Keywords

  • Bacilli-form virus
  • Brown shrimp
  • Carcinus maenas
  • Carcinus maenas nudivirus (CmNV)
  • Core genes
  • Crangon crangon
  • Crangon crangon nudivirus (CcNV)
  • Nudivirus
  • Shore crab
  • Taxonomy
  • Virus classifica-tion

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