TY - JOUR
T1 - Identification and full characterisation of two novel crustacean infecting members of the family nudiviridae provides support for two subfamilies
AU - Bateman, Kelly S.
AU - Kerr, Rose
AU - Stentiford, Grant D.
AU - Bean, Tim P.
AU - Hooper, Chantelle
AU - Van Eynde, Benigna
AU - Delbare, Daan
AU - Bojko, Jamie
AU - Christiaens, Olivier
AU - Taning, Clauvis N.T.
AU - Smagghe, Guy
AU - van Oers, Monique M.
AU - van Aerle, Ronny
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/8/26
Y1 - 2021/8/26
N2 - Multiple enveloped viruses with rod-shaped nucleocapsids have been described, infecting the epithelial cell nuclei within the hepatopancreas tubules of crustaceans. These bacilliform viruses share the ultrastructural characteristics of nudiviruses, a specific clade of viruses infecting arthropods. Using histology, electron microscopy and high throughput sequencing, we characterise two further bacilliform viruses from aquatic hosts, the brown shrimp (Crangon crangon) and the European shore crab (Carcinus maenas). We assembled the full double stranded, circular DNA genome sequences of these viruses (~113 and 132 kbp, respectively). Comparative genomics and phylogenetic analyses confirm that both belong within the family Nudiviridae but in separate clades representing nudiviruses found in freshwater and marine environments. We show that the three thymidine kinase (tk) genes present in all sequenced nudivirus genomes, thus far, were absent in the Crangon crangon nudivirus, suggesting there are twenty-eight core genes shared by all nudi-viruses. Furthermore, the phylogenetic data no longer support the subdivision of the family Nudi-viridae into four genera (Alphanudivirus to Deltanudivirus), as recently adopted by the International Committee on Taxonomy of Viruses (ICTV), but rather shows two main branches of the family that are further subdivided. Our data support a recent proposal to create two subfamilies within the family Nudiviridae, each subdivided into several genera.
AB - Multiple enveloped viruses with rod-shaped nucleocapsids have been described, infecting the epithelial cell nuclei within the hepatopancreas tubules of crustaceans. These bacilliform viruses share the ultrastructural characteristics of nudiviruses, a specific clade of viruses infecting arthropods. Using histology, electron microscopy and high throughput sequencing, we characterise two further bacilliform viruses from aquatic hosts, the brown shrimp (Crangon crangon) and the European shore crab (Carcinus maenas). We assembled the full double stranded, circular DNA genome sequences of these viruses (~113 and 132 kbp, respectively). Comparative genomics and phylogenetic analyses confirm that both belong within the family Nudiviridae but in separate clades representing nudiviruses found in freshwater and marine environments. We show that the three thymidine kinase (tk) genes present in all sequenced nudivirus genomes, thus far, were absent in the Crangon crangon nudivirus, suggesting there are twenty-eight core genes shared by all nudi-viruses. Furthermore, the phylogenetic data no longer support the subdivision of the family Nudi-viridae into four genera (Alphanudivirus to Deltanudivirus), as recently adopted by the International Committee on Taxonomy of Viruses (ICTV), but rather shows two main branches of the family that are further subdivided. Our data support a recent proposal to create two subfamilies within the family Nudiviridae, each subdivided into several genera.
KW - Bacilli-form virus
KW - Brown shrimp
KW - Carcinus maenas
KW - Carcinus maenas nudivirus (CmNV)
KW - Core genes
KW - Crangon crangon
KW - Crangon crangon nudivirus (CcNV)
KW - Nudivirus
KW - Shore crab
KW - Taxonomy
KW - Virus classifica-tion
U2 - 10.3390/v13091694
DO - 10.3390/v13091694
M3 - Article
AN - SCOPUS:85113937045
SN - 1999-4915
VL - 13
JO - Viruses
JF - Viruses
IS - 9
M1 - 1694
ER -