TY - JOUR
T1 - Identification and characterization of two RNA silencing suppressors encoded by ophioviruses
AU - Robles Luna, Gabriel
AU - Reyes, Carina A.
AU - Peña, Eduardo J.
AU - Ocolotobiche, Eliana
AU - Baeza, Cecilia
AU - Borniego, Maria Belén
AU - Kormelink, Richard
AU - García, María Laura
PY - 2017
Y1 - 2017
N2 - Citrus psorosis virus and Mirafiori lettuce big-vein virus are two members of the genus Ophiovirus, family Ophioviridae. So far, how these viruses can interfere in the antiviral RNA silencing pathway is not known. In this study, using a local GFP silencing assay on Nicotiana benthamiana, the 24K–25K and the movement protein (MP) of both viruses were identified as RNA silencing suppressor proteins. Upon their co-expression with GFP in N. benthamiana 16c plants, the proteins also showed to suppress systemic RNA (GFP) silencing. The MPCPsV and 24KCPsV proteins bind long (114 nucleotides) but not short-interfering (21 nt) dsRNA, and upon transgenic expression, plants showed developmental abnormalities that coincided with an altered miRNA accumulation pattern. Furthermore, both proteins were able to suppress miRNA-induced silencing of a GFP-sensor construct and the co-expression of MPCPsV and 24KCPsV exhibited a stronger effect, suggesting they act at different stages of the RNAi pathway.
AB - Citrus psorosis virus and Mirafiori lettuce big-vein virus are two members of the genus Ophiovirus, family Ophioviridae. So far, how these viruses can interfere in the antiviral RNA silencing pathway is not known. In this study, using a local GFP silencing assay on Nicotiana benthamiana, the 24K–25K and the movement protein (MP) of both viruses were identified as RNA silencing suppressor proteins. Upon their co-expression with GFP in N. benthamiana 16c plants, the proteins also showed to suppress systemic RNA (GFP) silencing. The MPCPsV and 24KCPsV proteins bind long (114 nucleotides) but not short-interfering (21 nt) dsRNA, and upon transgenic expression, plants showed developmental abnormalities that coincided with an altered miRNA accumulation pattern. Furthermore, both proteins were able to suppress miRNA-induced silencing of a GFP-sensor construct and the co-expression of MPCPsV and 24KCPsV exhibited a stronger effect, suggesting they act at different stages of the RNAi pathway.
KW - Citrus psorosis virus
KW - Mirafiori lettuce big-vein virus
KW - Ophiovirus
KW - RNA silencing
KW - Viral suppressor
U2 - 10.1016/j.virusres.2017.04.013
DO - 10.1016/j.virusres.2017.04.013
M3 - Article
SN - 0168-1702
VL - 235
SP - 96
EP - 105
JO - Virus Research
JF - Virus Research
ER -
