Identification and characterization of di- and tripeptide transporter DtpT of Listeria monocytogenes EGD-e

J.A. Wouters, T. Hain, A. Darji, E. Hüfner, H.H. Wemekamp-Kamphuis, T. Chakraborty, T. Abee

Research output: Contribution to journalArticleAcademicpeer-review

20 Citations (Scopus)

Abstract

Listeria monocytogenes is a gram-positive intracellular pathogen responsible for opportunistic infections in humans and animals. Here we identified and characterized the dtpT gene (lmo0555) of L. monocytogenes EGD-e, encoding the di- and tripeptide transporter, and assessed its role in growth under various environmental conditions as well as in the virulence of L. monocytogenes. Uptake of the dipeptide Pro-[14C]Ala was mediated by the DtpT transporter and was abrogated in a dtpT isogenic deletion mutant. The DtpT transporter was shown to be required for growth when the essential amino acids leucine and valine were supplied as peptides. The protective effect of glycine- and proline-containing peptides during growth in defined medium containing 3% NaCl was noted only in L. monocytogenes EGD-e, not in the dtpT mutant strain, indicating that the DtpT transporter is involved in salt stress protection. Infection studies showed that DtpT contributes to pathogenesis in a mouse infection model but has no role in bacterial growth following infection of J774 macrophages. These studies reveal that DptT may contribute to the virulence of L. monocytogenes
Original languageEnglish
Pages (from-to)5771-5778
JournalApplied and Environmental Microbiology
Volume71
Issue number10
DOIs
Publication statusPublished - 2005

Fingerprint

tripeptides
dipeptides
Dipeptides
Listeria monocytogenes
transporters
virulence
peptide
Growth
infection
Virulence
Infection
peptides
mutants
Peptides
Essential Amino Acids
Opportunistic Infections
Valine
valine
essential amino acids
glycine (amino acid)

Keywords

  • lactococcus-lactis
  • salt tolerance
  • minimal medium
  • peptide transporter
  • high osmolarity
  • growth
  • virulence
  • system
  • genes
  • protein

Cite this

Wouters, J.A. ; Hain, T. ; Darji, A. ; Hüfner, E. ; Wemekamp-Kamphuis, H.H. ; Chakraborty, T. ; Abee, T. / Identification and characterization of di- and tripeptide transporter DtpT of Listeria monocytogenes EGD-e. In: Applied and Environmental Microbiology. 2005 ; Vol. 71, No. 10. pp. 5771-5778.
@article{61bc15f90ffc4e8dba4db023b6786305,
title = "Identification and characterization of di- and tripeptide transporter DtpT of Listeria monocytogenes EGD-e",
abstract = "Listeria monocytogenes is a gram-positive intracellular pathogen responsible for opportunistic infections in humans and animals. Here we identified and characterized the dtpT gene (lmo0555) of L. monocytogenes EGD-e, encoding the di- and tripeptide transporter, and assessed its role in growth under various environmental conditions as well as in the virulence of L. monocytogenes. Uptake of the dipeptide Pro-[14C]Ala was mediated by the DtpT transporter and was abrogated in a dtpT isogenic deletion mutant. The DtpT transporter was shown to be required for growth when the essential amino acids leucine and valine were supplied as peptides. The protective effect of glycine- and proline-containing peptides during growth in defined medium containing 3{\%} NaCl was noted only in L. monocytogenes EGD-e, not in the dtpT mutant strain, indicating that the DtpT transporter is involved in salt stress protection. Infection studies showed that DtpT contributes to pathogenesis in a mouse infection model but has no role in bacterial growth following infection of J774 macrophages. These studies reveal that DptT may contribute to the virulence of L. monocytogenes",
keywords = "lactococcus-lactis, salt tolerance, minimal medium, peptide transporter, high osmolarity, growth, virulence, system, genes, protein",
author = "J.A. Wouters and T. Hain and A. Darji and E. H{\"u}fner and H.H. Wemekamp-Kamphuis and T. Chakraborty and T. Abee",
year = "2005",
doi = "10.1128/AEM.71.10.5771-5778.2005",
language = "English",
volume = "71",
pages = "5771--5778",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "10",

}

Identification and characterization of di- and tripeptide transporter DtpT of Listeria monocytogenes EGD-e. / Wouters, J.A.; Hain, T.; Darji, A.; Hüfner, E.; Wemekamp-Kamphuis, H.H.; Chakraborty, T.; Abee, T.

In: Applied and Environmental Microbiology, Vol. 71, No. 10, 2005, p. 5771-5778.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Identification and characterization of di- and tripeptide transporter DtpT of Listeria monocytogenes EGD-e

AU - Wouters, J.A.

AU - Hain, T.

AU - Darji, A.

AU - Hüfner, E.

AU - Wemekamp-Kamphuis, H.H.

AU - Chakraborty, T.

AU - Abee, T.

PY - 2005

Y1 - 2005

N2 - Listeria monocytogenes is a gram-positive intracellular pathogen responsible for opportunistic infections in humans and animals. Here we identified and characterized the dtpT gene (lmo0555) of L. monocytogenes EGD-e, encoding the di- and tripeptide transporter, and assessed its role in growth under various environmental conditions as well as in the virulence of L. monocytogenes. Uptake of the dipeptide Pro-[14C]Ala was mediated by the DtpT transporter and was abrogated in a dtpT isogenic deletion mutant. The DtpT transporter was shown to be required for growth when the essential amino acids leucine and valine were supplied as peptides. The protective effect of glycine- and proline-containing peptides during growth in defined medium containing 3% NaCl was noted only in L. monocytogenes EGD-e, not in the dtpT mutant strain, indicating that the DtpT transporter is involved in salt stress protection. Infection studies showed that DtpT contributes to pathogenesis in a mouse infection model but has no role in bacterial growth following infection of J774 macrophages. These studies reveal that DptT may contribute to the virulence of L. monocytogenes

AB - Listeria monocytogenes is a gram-positive intracellular pathogen responsible for opportunistic infections in humans and animals. Here we identified and characterized the dtpT gene (lmo0555) of L. monocytogenes EGD-e, encoding the di- and tripeptide transporter, and assessed its role in growth under various environmental conditions as well as in the virulence of L. monocytogenes. Uptake of the dipeptide Pro-[14C]Ala was mediated by the DtpT transporter and was abrogated in a dtpT isogenic deletion mutant. The DtpT transporter was shown to be required for growth when the essential amino acids leucine and valine were supplied as peptides. The protective effect of glycine- and proline-containing peptides during growth in defined medium containing 3% NaCl was noted only in L. monocytogenes EGD-e, not in the dtpT mutant strain, indicating that the DtpT transporter is involved in salt stress protection. Infection studies showed that DtpT contributes to pathogenesis in a mouse infection model but has no role in bacterial growth following infection of J774 macrophages. These studies reveal that DptT may contribute to the virulence of L. monocytogenes

KW - lactococcus-lactis

KW - salt tolerance

KW - minimal medium

KW - peptide transporter

KW - high osmolarity

KW - growth

KW - virulence

KW - system

KW - genes

KW - protein

U2 - 10.1128/AEM.71.10.5771-5778.2005

DO - 10.1128/AEM.71.10.5771-5778.2005

M3 - Article

VL - 71

SP - 5771

EP - 5778

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 10

ER -