TY - JOUR
T1 - Identification and characterization of a novel tospovirus species using a new RT-PCR approach
AU - Cortez, I.
AU - Saaijer, J.
AU - Wonjkaew, K.S.
AU - Pereira, A.M.
AU - Goldbach, R.W.
AU - Peters, D.
AU - Kormelink, R.
PY - 2001
Y1 - 2001
N2 - Summary. A novel tospovirus serologically distinct from all established tospo- virus species was found in Thailand in Physalis minima L. The S RNA of this virus was cloned by a new RT-PCR approach revealing a nucleotide sequence of 3257 nucleotides. The ambisense RNA segment encoded a nonstructural protein (NSs) of 469 amino acids, with a predicted Mr of 53.2 kDa, and a nucleoprotein (N) of 279 amino acids and a Mr of 31.0 kDa, so far the largest N protein known for any tospovirus species. N protein sequence comparisons revealed closest relationship to the species Watermelon bud necrosis virus (58 dentity), Watermelon silver mottle virus and Peanut bud necrosis virus (57€and a distant relationship to Peanut yellow spot virus (23€and Peanut chlorotic fanspot virus (22Ž
AB - Summary. A novel tospovirus serologically distinct from all established tospo- virus species was found in Thailand in Physalis minima L. The S RNA of this virus was cloned by a new RT-PCR approach revealing a nucleotide sequence of 3257 nucleotides. The ambisense RNA segment encoded a nonstructural protein (NSs) of 469 amino acids, with a predicted Mr of 53.2 kDa, and a nucleoprotein (N) of 279 amino acids and a Mr of 31.0 kDa, so far the largest N protein known for any tospovirus species. N protein sequence comparisons revealed closest relationship to the species Watermelon bud necrosis virus (58 dentity), Watermelon silver mottle virus and Peanut bud necrosis virus (57€and a distant relationship to Peanut yellow spot virus (23€and Peanut chlorotic fanspot virus (22Ž
U2 - 10.1007/s007050170174
DO - 10.1007/s007050170174
M3 - Article
SN - 0304-8608
VL - 146
SP - 265
EP - 278
JO - Archives of Virology
JF - Archives of Virology
ER -