Identification and characterization of a novel tospovirus species using a new RT-PCR approach

I. Cortez, J. Saaijer, K.S. Wonjkaew, A.M. Pereira, R.W. Goldbach, D. Peters, R. Kormelink

Research output: Contribution to journalArticleAcademicpeer-review

59 Citations (Scopus)

Abstract

Summary. A novel tospovirus serologically distinct from all established tospo- virus species was found in Thailand in Physalis minima L. The S RNA of this virus was cloned by a new RT-PCR approach revealing a nucleotide sequence of 3257 nucleotides. The ambisense RNA segment encoded a nonstructural protein (NSs) of 469 amino acids, with a predicted Mr of 53.2 kDa, and a nucleoprotein (N) of 279 amino acids and a Mr of 31.0 kDa, so far the largest N protein known for any tospovirus species. N protein sequence comparisons revealed closest relationship to the species Watermelon bud necrosis virus (58 dentity), Watermelon silver mottle virus and Peanut bud necrosis virus (57€and a distant relationship to Peanut yellow spot virus (23€and Peanut chlorotic fanspot virus (22Ž
Original languageEnglish
Pages (from-to)265-278
JournalArchives of Virology
Volume146
DOIs
Publication statusPublished - 2001

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