Identification and characterization of a new class of Tomato spotted wilt virus isolates that break Tsw-based resistance in a temperature-dependent manner

D. de Ronde, D. Lohuis, R. Kormelink*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

The single dominant Tsw resistance gene from Capsicum chinense against the Tomato spotted wilt orthotospovirus (TSWV) is temperature sensitive, i.e. the resistance fails to function at or above 32 °C. This study describes a new class of temperature-sensitive resistance-breaking TSWV isolates that induce Tsw-mediated resistance at T < 28 °C but at T ≥ 28 °C break this resistance. The NSs genes from these isolates were cloned and expressed to be analysed for RNA silencing suppressor (RSS) activity and the ability to induce a Tsw-mediated hypersensitive response (HR) in C. chinense and Capsicum annuum (Tsw+). Unlike in viral infection, transient expression of some of the NSs proteins at standard temperatures (22 °C) did not induce Tsw-mediated HR, although varying degrees of RSS activity were observed. Attempts to express and test the NSs proteins for functionality at an elevated temperature through agroinfiltration remained unsuccessful. The NSs proteins of some TSWV resistance-breaking (RB) isolates were analysed and found to lack amino acid residues that were previously shown to be important for RNA silencing suppression and avirulence. This study describes a new class of resistance-breaking TSWV isolates that may be of importance for breeders and growers and for which the underlying mechanism still remains unknown.

Original languageEnglish
Pages (from-to)60-71
JournalPlant Pathology
Volume68
Issue number1
Early online date3 Oct 2018
DOIs
Publication statusPublished - Jan 2019

Keywords

  • Capsicum
  • NSs protein
  • RNA-silencing suppressor
  • temperature-sensitive resistance-breaking
  • Tomato spotted wilt virus
  • Tsw-based resistance

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