Identification and characterization of a DNA photolyase-containing baculovirus from Chrysodeixis chalcites

M.M. van Oers, E.A. Herniou, M. Usmany, G.J. Messelink, J.M. Vlak

Research output: Contribution to journalArticleAcademicpeer-review

30 Citations (Scopus)


A hitherto unknown single nucleocapsid nucleopolyhedrovirus (SNPV) with a unique property was isolated from larvae of the looper Chrysodeixis chalcites (Lepidoptera, Noctuidae, Plusiinae). Polyhedrin, lef-8, and pif-2 gene sequences were obtained by PCR with degenerate primers and used for phylogenetic analysis. ChchNPV belonged to class II NPVs and its polyhedrin sequence was most similar to that of class II NPVs of other members of the subfamily Plusiinae. Further genetic characterization involved the random cloning of HindIII fragments into a plasmid vector and analysis by end-in sequencing. A gene so far unique to baculoviruses was identified, which encodes a putative DNA repair enzyme: cyclobutane pyrimidine dimer (CPD) DNA photolyase (dpl). The transcriptional activity of this gene was demonstrated in both ChchNPV-infected C. chalcites larvae and infected Trichoplusia W High Five cells by RT-PCR and 5' and 3' RACE analysis. The possible role of this gene in the biology of the virus is discussed. (C) 2004 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)460-470
Number of pages11
Issue number2
Publication statusPublished - 2004


  • nuclear polyhedrosis-virus
  • envelope fusion protein
  • genome sequence
  • fowlpox virus
  • repair enzyme
  • gene
  • nucleopolyhedrovirus
  • expression
  • substitution
  • glycosylase


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