Identification and characterisation of Tomato torrado virus, a new plant picorna-like virus from tomato

M. Verbeek, A.M. Dullemans, J.F.J.M. van den Heuvel, P.C. Maris, R.A.A. van der Vlugt

    Research output: Contribution to journalArticleAcademicpeer-review

    61 Citations (Scopus)

    Abstract

    A new virus was isolated from tomato plants from the Murcia region in Spain which showed symptoms of 'torrado disease'; very distinct necrotic, almost burn-like symptoms on leaves of infected plants. The virus particles are isometric with a diameter of approximately 28 nm. The viral genome consists of two (+)ssRNA molecules of 7793 (RNA1) and 5389 nts (RNA2). RNA1 contains one open reading frame (ORF) encoding a predicted polyprotein of 241 kDa that shows conserved regions with motifs typical for a protease-cofactor, a helicase, a protease and an RNA-dependent RNA polymerase. RNA2 contains two, partially overlapping ORFs potentially encoding proteins of 20 and 134 kDa. These viral RNAs are encapsidated by three proteins with estimated sizes of 35, 26 and 23 kDa. Direct protein sequencing mapped these coat proteins to ORF2 on RNA2. Phylogenetic analyses of nucleotide and derived amino acid sequences showed that the virus is related to but distinct from viruses belonging to the genera Sequivirus, Sadwavirus and Cheravirus. This new virus, for which the name tomato torrado virus is proposed, most likely represents a member of a new plant virus genus
    Original languageEnglish
    Pages (from-to)881-890
    JournalArchives of Virology
    Volume152
    Issue number5
    DOIs
    Publication statusPublished - 2007

    Fingerprint

    Lycopersicon esculentum
    Viruses
    Sequivirus
    Open Reading Frames
    Peptide Hydrolases
    RNA Replicase
    Plant Viruses
    Polyproteins
    Plant Leaves
    Viral Genome
    Protein Sequence Analysis
    Viral RNA
    Capsid Proteins
    Burns
    Virion
    Spain
    Names
    Amino Acid Sequence
    Proteins
    Nucleotides

    Keywords

    • rasp-leaf-virus
    • nucleotide-sequence analysis
    • flat apple isolate
    • rna viruses
    • polymerases
    • trypsin
    • family

    Cite this

    @article{6be28570513543779021952bff4f2cd9,
    title = "Identification and characterisation of Tomato torrado virus, a new plant picorna-like virus from tomato",
    abstract = "A new virus was isolated from tomato plants from the Murcia region in Spain which showed symptoms of 'torrado disease'; very distinct necrotic, almost burn-like symptoms on leaves of infected plants. The virus particles are isometric with a diameter of approximately 28 nm. The viral genome consists of two (+)ssRNA molecules of 7793 (RNA1) and 5389 nts (RNA2). RNA1 contains one open reading frame (ORF) encoding a predicted polyprotein of 241 kDa that shows conserved regions with motifs typical for a protease-cofactor, a helicase, a protease and an RNA-dependent RNA polymerase. RNA2 contains two, partially overlapping ORFs potentially encoding proteins of 20 and 134 kDa. These viral RNAs are encapsidated by three proteins with estimated sizes of 35, 26 and 23 kDa. Direct protein sequencing mapped these coat proteins to ORF2 on RNA2. Phylogenetic analyses of nucleotide and derived amino acid sequences showed that the virus is related to but distinct from viruses belonging to the genera Sequivirus, Sadwavirus and Cheravirus. This new virus, for which the name tomato torrado virus is proposed, most likely represents a member of a new plant virus genus",
    keywords = "rasp-leaf-virus, nucleotide-sequence analysis, flat apple isolate, rna viruses, polymerases, trypsin, family",
    author = "M. Verbeek and A.M. Dullemans and {van den Heuvel}, J.F.J.M. and P.C. Maris and {van der Vlugt}, R.A.A.",
    year = "2007",
    doi = "10.1007/s00705-006-0917-6",
    language = "English",
    volume = "152",
    pages = "881--890",
    journal = "Archives of Virology",
    issn = "0304-8608",
    publisher = "Springer Verlag",
    number = "5",

    }

    Identification and characterisation of Tomato torrado virus, a new plant picorna-like virus from tomato. / Verbeek, M.; Dullemans, A.M.; van den Heuvel, J.F.J.M.; Maris, P.C.; van der Vlugt, R.A.A.

    In: Archives of Virology, Vol. 152, No. 5, 2007, p. 881-890.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Identification and characterisation of Tomato torrado virus, a new plant picorna-like virus from tomato

    AU - Verbeek, M.

    AU - Dullemans, A.M.

    AU - van den Heuvel, J.F.J.M.

    AU - Maris, P.C.

    AU - van der Vlugt, R.A.A.

    PY - 2007

    Y1 - 2007

    N2 - A new virus was isolated from tomato plants from the Murcia region in Spain which showed symptoms of 'torrado disease'; very distinct necrotic, almost burn-like symptoms on leaves of infected plants. The virus particles are isometric with a diameter of approximately 28 nm. The viral genome consists of two (+)ssRNA molecules of 7793 (RNA1) and 5389 nts (RNA2). RNA1 contains one open reading frame (ORF) encoding a predicted polyprotein of 241 kDa that shows conserved regions with motifs typical for a protease-cofactor, a helicase, a protease and an RNA-dependent RNA polymerase. RNA2 contains two, partially overlapping ORFs potentially encoding proteins of 20 and 134 kDa. These viral RNAs are encapsidated by three proteins with estimated sizes of 35, 26 and 23 kDa. Direct protein sequencing mapped these coat proteins to ORF2 on RNA2. Phylogenetic analyses of nucleotide and derived amino acid sequences showed that the virus is related to but distinct from viruses belonging to the genera Sequivirus, Sadwavirus and Cheravirus. This new virus, for which the name tomato torrado virus is proposed, most likely represents a member of a new plant virus genus

    AB - A new virus was isolated from tomato plants from the Murcia region in Spain which showed symptoms of 'torrado disease'; very distinct necrotic, almost burn-like symptoms on leaves of infected plants. The virus particles are isometric with a diameter of approximately 28 nm. The viral genome consists of two (+)ssRNA molecules of 7793 (RNA1) and 5389 nts (RNA2). RNA1 contains one open reading frame (ORF) encoding a predicted polyprotein of 241 kDa that shows conserved regions with motifs typical for a protease-cofactor, a helicase, a protease and an RNA-dependent RNA polymerase. RNA2 contains two, partially overlapping ORFs potentially encoding proteins of 20 and 134 kDa. These viral RNAs are encapsidated by three proteins with estimated sizes of 35, 26 and 23 kDa. Direct protein sequencing mapped these coat proteins to ORF2 on RNA2. Phylogenetic analyses of nucleotide and derived amino acid sequences showed that the virus is related to but distinct from viruses belonging to the genera Sequivirus, Sadwavirus and Cheravirus. This new virus, for which the name tomato torrado virus is proposed, most likely represents a member of a new plant virus genus

    KW - rasp-leaf-virus

    KW - nucleotide-sequence analysis

    KW - flat apple isolate

    KW - rna viruses

    KW - polymerases

    KW - trypsin

    KW - family

    U2 - 10.1007/s00705-006-0917-6

    DO - 10.1007/s00705-006-0917-6

    M3 - Article

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    SP - 881

    EP - 890

    JO - Archives of Virology

    JF - Archives of Virology

    SN - 0304-8608

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