Hyperhydricity

Underlying mechanisms

G.J.M. de Klerk*, N. van den Dries, F.A. Krens

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

The apoplast consists of the cell wall continuum and the intercellular spaces. Flooding of the apoplast is the major causative factor of the hyperhydricity (HH) syndrome. Flooding reduces gas exchange by cells. This leads to anaerobiosis and to accumulation within cells of gases like ethylene. The flooding is caused by the ample availability of water in tissue culture and occurs more on liquid medium and Gelritesolidified medium than on agar-solidified medium. Gelrite causes more HH since medium solidified with Gelrite liquefies, probably because of chelating compounds excreted by plant tissues. Root pressure causes translocation of water from the roots to the apoplast. At the same time, the water that has entered the apoplast cannot easily be removed in vitro by transpiration due to the very high relative humidity in the headspace. When the stomata are forced to close by addition of salicylic acid, transpiration is even more impaired. This results in an increase of HH.

Original languageEnglish
Pages (from-to)269-275
JournalActa Horticulturae
Volume1155
DOIs
Publication statusPublished - 2017
EventVI International Symposium on Production and Establishment of Micropropagated Plants - Sanremo, Italy
Duration: 19 May 201524 May 2015

Fingerprint

hyperhydricity
apoplast
transpiration
root pressure
intercellular spaces
water
anaerobiosis
salicylic acid
headspace analysis
stomata
gas exchange
tissue culture
plant tissues
ethylene
relative humidity
agar
cell walls
gases
cells
liquids

Keywords

  • Apoplast
  • Arabidopsis
  • Flooding of the apoplast
  • Intercellular spaces
  • Vitrification

Cite this

de Klerk, G.J.M. ; van den Dries, N. ; Krens, F.A. / Hyperhydricity : Underlying mechanisms. In: Acta Horticulturae. 2017 ; Vol. 1155. pp. 269-275.
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Hyperhydricity : Underlying mechanisms. / de Klerk, G.J.M.; van den Dries, N.; Krens, F.A.

In: Acta Horticulturae, Vol. 1155, 2017, p. 269-275.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Hyperhydricity

T2 - Underlying mechanisms

AU - de Klerk, G.J.M.

AU - van den Dries, N.

AU - Krens, F.A.

PY - 2017

Y1 - 2017

N2 - The apoplast consists of the cell wall continuum and the intercellular spaces. Flooding of the apoplast is the major causative factor of the hyperhydricity (HH) syndrome. Flooding reduces gas exchange by cells. This leads to anaerobiosis and to accumulation within cells of gases like ethylene. The flooding is caused by the ample availability of water in tissue culture and occurs more on liquid medium and Gelritesolidified medium than on agar-solidified medium. Gelrite causes more HH since medium solidified with Gelrite liquefies, probably because of chelating compounds excreted by plant tissues. Root pressure causes translocation of water from the roots to the apoplast. At the same time, the water that has entered the apoplast cannot easily be removed in vitro by transpiration due to the very high relative humidity in the headspace. When the stomata are forced to close by addition of salicylic acid, transpiration is even more impaired. This results in an increase of HH.

AB - The apoplast consists of the cell wall continuum and the intercellular spaces. Flooding of the apoplast is the major causative factor of the hyperhydricity (HH) syndrome. Flooding reduces gas exchange by cells. This leads to anaerobiosis and to accumulation within cells of gases like ethylene. The flooding is caused by the ample availability of water in tissue culture and occurs more on liquid medium and Gelritesolidified medium than on agar-solidified medium. Gelrite causes more HH since medium solidified with Gelrite liquefies, probably because of chelating compounds excreted by plant tissues. Root pressure causes translocation of water from the roots to the apoplast. At the same time, the water that has entered the apoplast cannot easily be removed in vitro by transpiration due to the very high relative humidity in the headspace. When the stomata are forced to close by addition of salicylic acid, transpiration is even more impaired. This results in an increase of HH.

KW - Apoplast

KW - Arabidopsis

KW - Flooding of the apoplast

KW - Intercellular spaces

KW - Vitrification

U2 - 10.17660/ActaHortic.2017.1155.39

DO - 10.17660/ActaHortic.2017.1155.39

M3 - Article

VL - 1155

SP - 269

EP - 275

JO - Acta Horticulturae

JF - Acta Horticulturae

SN - 0567-7572

ER -