Hydroperoxide production from linoleic acid by heterologous Gaeumannomyces graminis tritici lipoxygenase: Optimization and scale-up

J.J. Villaverde, V. van der Vlist, S.A.O. Santos, T. Haarmann, K. Langfelder, M. Pirttimaa, A. Nyyssola, S. Jylhä, T. Tamminen, K. Kruus, L.H. de Graaff, C. Pascoal Neto, M.M.Q. Simoes, M.R.M. Domingues, A.J.D. Silvestre, J. Eidner, J. Buchert

Research output: Contribution to journalArticleAcademicpeer-review

21 Citations (Scopus)

Abstract

Linoleic acid was converted into hydroperoxides by a Gaeumannomyces graminis tritici lipoxygenase produced recombinantly in Trichoderma reesei. Hydroperoxide production was optimized using a face-centred experimental design in order to study the effects of pH, temperature and time on the conversion of linoleic acid into four regioisomeric hydroperoxyoctadecadienoic acids (HPODE): 13-(Z,E)-, 9-(E,Z)-, 13-(E,E)-, 9-(E,E)-HPODE. Fitting equations described satisfactorily the system behavior and showed that reaction time was the most influencing independent variable. A set of independent variables (pH = 6.7, temperature = 23.9 °C and time = 18 h) allowed to obtain high yields of hydroperoxides (88.0%) with a good selectivity for the 13-(Z,E)-HPODE isomer (47.4%) when the initial substrate concentration was 10 g/L. The production was further investigated using industrially relevant linoleic acid concentrations (100–300 g/L) leading to HPODE yields of ~40% and the volumetric productivity 3.6 g/(L h), and a selectivity for 13-(Z,E)-HPODE of around 74%
Original languageEnglish
Pages (from-to)82-90
JournalChemical Engineering Journal
Volume217
DOIs
Publication statusPublished - 2013

Keywords

  • unsaturated fatty-acids
  • soybean lipoxygenase
  • immobilized lipoxygenase
  • manganese lipoxygenase
  • mass-spectrometry
  • oxidation
  • identification
  • system
  • state
  • oil

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