Hydrolysates of glycated and heat treated peanut 7S globulin (Ara h 1) modulate human gut microbiota proliferation, survival and adhesion

M. Teodorowicz, D. Swiatecka, H.F.J. Savelkoul, H.J. Wichers, E. Kostyra

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

Aims - Evaluation of an effect of glycation of Ara h 1 on proliferation and survival rate and adhesion of intestinal Enterococcus faecalis, Escherichia coli and Lactobacillus acidophilus. Methods and Results - Pure Ara h 1 heated at three different temperature conditions (G37°C, G60°C and C145°C) in the presence or absence of glucose was subjected to enzymatic hydrolysis. Impacts of Ara h 1 hydrolysates on the bacterial proliferation, survival rate and adhesion to Caco-2 cells in mono and heterogeneous cultures were studied with fluorescent techniques: DAPI, LIVE/DEAD staining and FISH. Examined hydrolysates hindered proliferation of E. coli and E. faecalis with simultaneous decrease in their survival. Maillard reaction (MR, glycation) of Ara h 1 did not alter the effect of hydrolysates on bacterial proliferation rate. Hydrolysates modified at 60°C and 145°C with glucose altered the profile of immobilized bacteria, mostly by lowering the number of adhering E. coli and promoting the adhesion of bacteria from genera Lactobacillus and Enterococcus. Conclusions - Ara h1 hydrolysates processed in various ways demonstrated their strong modulatory effect on bacterial proliferation, survival rate and adhesion. Significance and Impact of Study - Reducing the adhesion of opportunistic bacteria by hydrolysates of Ara h 1 glycated at 60°C and 145°C, together with modulation of immobilization of beneficial lactobacilli and enterococci, may be of relevance in terms of the physiological status of the intestinal barrier
Original languageEnglish
Pages (from-to)424-434
JournalJournal of Applied Microbiology
Volume116
Issue number2
DOIs
Publication statusPublished - 2014

Keywords

  • maillard reaction-products
  • intestinal microbiota
  • in-vitro
  • proteins
  • health
  • allergen
  • differentiation
  • prevalence
  • chemistry
  • barrier

Fingerprint Dive into the research topics of 'Hydrolysates of glycated and heat treated peanut 7S globulin (Ara h 1) modulate human gut microbiota proliferation, survival and adhesion'. Together they form a unique fingerprint.

  • Cite this