Humic Acid-Like and Fulvic Acid-Like Inhibition on the Hydrolysis of Cellulose and Tributyrin

T.V. Fernandes, J.B. van Lier, Grietje Zeeman

Research output: Contribution to journalArticleAcademicpeer-review

49 Citations (Scopus)

Abstract

Enzymatic hydrolysis of complex wastes is a critical step for efficient biogas production in anaerobic digesters. Inhibition of this hydrolytic step was studied by addition of humic acid-like (HAL) and fulvic acid-like (FAL) substances, extracted from maize silage and fresh cow manure, to batch tests with cellulose and cellulases from Aspergillus niger and tributyrin and lipases from Candida rugosa. To confirm the inhibition results, microbially catalysed hydrolysis trials were performed, where Fibrobacter succinogenes was grown exclusively on cellulose. Hydrolysis products, as glucose, glycerol and butyric acid, were measured to determine the hydrolysis efficiency and its rate. Cellulose hydrolysis was inhibited by 0.5 to 5.0 g l−1 of HAL and FAL substances extracted from maize and cow manure, which are relevant concentrations for manure and maize digestion. Tributyrin hydrolysis, on the other hand, was only inhibited by 0.5 to 5.0 g l−1 of HAL substances extracted from maize and cow manure and not by FAL substances.

Original languageEnglish
Pages (from-to)821-831
Number of pages11
JournalBio Energy Research
Volume8
Issue number2
DOIs
Publication statusPublished - 1 Jun 2015

Keywords

  • Anaerobic hydrolysis inhibition
  • Cellulose
  • Humic and fulvic acids
  • Lipids
  • Repolymerisation

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