Human T47D-ERß breast cancer cells with tetracycline-dependent ERß expression reflect ERa/ERß ratios in rat and human breast tissue

N.M. Evers, T.M.C. van de Klundert, Y.M. van Aesch, S. Wang, W.K. de Roos, A. Romano, L.H.J. de Haan, A.J. Murk, A.G.H. Ederveen, I.M.C.M. Rietjens, J.P. Groten

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

T47D-ER beta breast cancer cells with tetracycline-dependent ER beta expression and constant ER alpha expression can be used to investigate effects of varying ER alpha/ER beta ratios on estrogen-induced cellular responses. This study defines conditions at which ER alpha/ER beta ratios in T47D-ER beta cells best mimic ER alpha/ER beta ratios in breast and other estrogen-sensitive tissues in vivo in rat as well as in human. Protein and mRNA levels of ER alpha and ER beta were analyzed in T47D-ER beta cells exposed to a range of tetracycline concentrations and compared to ER alpha and ER beta levels found in breast, prostate, and uterus from rat and human origin. The ER alpha/ER beta ratio in T47D-ER beta cells exposed to >150 ng/ml tetracycline is comparable to the ratio found in rat mammary gland and in human breast tissue. The ER alpha/ER beta ratio of other estrogen-sensitive rat and human tissues can also be mimicked in T47D-ER beta cells. The ER alpha/ER beta ratio found in MCF-7 and native T47D breast cancer cell lines did not reflect ratios in analyzed rat and human tissues, which further supports the use of T47D-ER beta cells as model for estrogen-responsive tissues. Using 17 beta-estradiol and the T47D-ER beta cells under the conditions defined to mimic various tissues it could be demonstrated how these different tissues vary in their proliferative response. (C) 2013 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)1753-1761
Number of pages9
JournalToxicology in Vitro
Volume27
Issue number6
DOIs
Publication statusPublished - 2013

Keywords

  • estrogen-receptor-beta
  • ovarian carcinogenesis
  • clinical-application
  • reproductive-tract
  • osteosarcoma cells
  • gene-expression
  • human prostate
  • messenger-rna
  • c-fos
  • growth

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