Human intestinal and lung cell lines exposed to beta-carotene show a large variation in intracellular levels of beta-carotene and its metabolites

N.L.W. van Franssen-Hal, J.E. Bunschoten, D.P. Venema, P.C.H. Hollman, G. Riss, J. Keijer

    Research output: Contribution to journalArticleAcademicpeer-review

    10 Citations (Scopus)

    Abstract

    Although in vitro models are often used in ß-carotene research, knowledge about the uptake and metabolism of ß-carotene in cell lines is lacking. We measured by HPLC the intracellular levels of ß-carotene and its metabolites in 9 human intestinal and lung cell lines after exposure to 1 ¿M ß-carotene during 2, 6, 30, 54 h, and 3 weeks. In three colorectal carcinoma cell lines only low levels of ß-carotene could be detected and an apparent linear increase in intracellular ß-carotene was observed during the whole exposure period of 3 weeks. The remaining cell lines (an SV40 transformed colon cell line, a small intestinal carcinoma cell line and several lung cell lines) had medium or high intracellular ß-carotene levels. In these cell lines intracellular ß-carotene quickly increased during the first 54 h of exposure and after 3 weeks no further increase was observed, suggesting a stable level of ß-carotene after 54 h. Estimated intracellular concentrations at steady-state levels varied between 2 and 5 ¿M (low) or 9 and 55 ¿M (medium/high). Our results seem to indicate that an active uptake mechanism of ß-carotene exists in at least a subset of cell lines. Seven different ß-carotene metabolites were detected in the various cell lines (cis-carotene, retinol, three epoxy-carotenes, and two retinyl esters). Metabolite levels were the highest in cells with medium or high ß-carotene levels. Each cell line appeared to have a distinct metabolite profile. No intestinal or lung specific pattern could be found, but two epoxy-carotene metabolites were not detectable in the colon cell lines
    Original languageEnglish
    Pages (from-to)32-41
    JournalArchives of Biochemistry and Biophysics
    Volume439
    Issue number1
    DOIs
    Publication statusPublished - 2005

    Keywords

    • lecithin-retinol acyltransferase
    • beta,beta-carotene 15,15'-dioxygenase
    • excentric cleavage
    • in-vitro
    • vitamin
    • acid
    • expression
    • oxidation
    • enzymes
    • ferret

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