HPLC inorganic arsenic speciation analysis of samples containing high sulfuric acid and iron levels

P.A. Gonzalez-Contreras, I.P.A.M. Gerrits, J. Weijma, C.J.N. Buisman

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

To monitor the oxidation of arsenite to arsenate in oxidizing and bioleaching reactors, speciation analysis of the inorganic arsenic compounds is required. Existing arsenic speciation analysis techniques are based on the use of liquid chromatography columns coupled to detector equipment such as inductively coupled plasma optical emission spectrometry (ICP-OES). These techniques are fairly complex and expensive. Therefore, for analysis of arsenic speciation in our studies on thermophilic iron and arsenic oxidation, we developed a simple (and inexpensive) method based on anionic ion exclusion chromatography using sulfuric acid as mobile phase, followed by UV detection. The detection limit was 0.1¿mg¿L-1 of arsenite (As3+) and 0.1¿mg¿L-1 of arsenate (As5+). The method was validated with samples from an iron-oxidizing bioreactor containing 0.12¿mol¿L-1 H2SO4, 1¿g¿L-1 arsenic, and 3¿g¿L-1 iron. Samples from arsenopyritic gold concentrates by partial bio-oxidation and leaching samples were analyzed, showing the usefulness of the method for arsenic contained in environmental compartments. We also describe the apparent effect of the arsenic valence state on ICP-OES detection for total arsenic.
Original languageEnglish
Pages (from-to)415-423
JournalToxicological & Environmental Chemistry
Volume93
Issue number3
DOIs
Publication statusPublished - 2011

Fingerprint

Arsenic
Sulfuric acid
sulfuric acid
arsenic
Iron
High Pressure Liquid Chromatography
iron
Inductively coupled plasma
arsenite
Oxidation
arsenate
Spectrometry
oxidation
Spectrum Analysis
Arsenic compounds
spectrometry
Arsenicals
Bioleaching
plasma
Liquid chromatography

Keywords

  • icp-oes
  • spectrometry

Cite this

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title = "HPLC inorganic arsenic speciation analysis of samples containing high sulfuric acid and iron levels",
abstract = "To monitor the oxidation of arsenite to arsenate in oxidizing and bioleaching reactors, speciation analysis of the inorganic arsenic compounds is required. Existing arsenic speciation analysis techniques are based on the use of liquid chromatography columns coupled to detector equipment such as inductively coupled plasma optical emission spectrometry (ICP-OES). These techniques are fairly complex and expensive. Therefore, for analysis of arsenic speciation in our studies on thermophilic iron and arsenic oxidation, we developed a simple (and inexpensive) method based on anionic ion exclusion chromatography using sulfuric acid as mobile phase, followed by UV detection. The detection limit was 0.1¿mg¿L-1 of arsenite (As3+) and 0.1¿mg¿L-1 of arsenate (As5+). The method was validated with samples from an iron-oxidizing bioreactor containing 0.12¿mol¿L-1 H2SO4, 1¿g¿L-1 arsenic, and 3¿g¿L-1 iron. Samples from arsenopyritic gold concentrates by partial bio-oxidation and leaching samples were analyzed, showing the usefulness of the method for arsenic contained in environmental compartments. We also describe the apparent effect of the arsenic valence state on ICP-OES detection for total arsenic.",
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HPLC inorganic arsenic speciation analysis of samples containing high sulfuric acid and iron levels. / Gonzalez-Contreras, P.A.; Gerrits, I.P.A.M.; Weijma, J.; Buisman, C.J.N.

In: Toxicological & Environmental Chemistry, Vol. 93, No. 3, 2011, p. 415-423.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - HPLC inorganic arsenic speciation analysis of samples containing high sulfuric acid and iron levels

AU - Gonzalez-Contreras, P.A.

AU - Gerrits, I.P.A.M.

AU - Weijma, J.

AU - Buisman, C.J.N.

PY - 2011

Y1 - 2011

N2 - To monitor the oxidation of arsenite to arsenate in oxidizing and bioleaching reactors, speciation analysis of the inorganic arsenic compounds is required. Existing arsenic speciation analysis techniques are based on the use of liquid chromatography columns coupled to detector equipment such as inductively coupled plasma optical emission spectrometry (ICP-OES). These techniques are fairly complex and expensive. Therefore, for analysis of arsenic speciation in our studies on thermophilic iron and arsenic oxidation, we developed a simple (and inexpensive) method based on anionic ion exclusion chromatography using sulfuric acid as mobile phase, followed by UV detection. The detection limit was 0.1¿mg¿L-1 of arsenite (As3+) and 0.1¿mg¿L-1 of arsenate (As5+). The method was validated with samples from an iron-oxidizing bioreactor containing 0.12¿mol¿L-1 H2SO4, 1¿g¿L-1 arsenic, and 3¿g¿L-1 iron. Samples from arsenopyritic gold concentrates by partial bio-oxidation and leaching samples were analyzed, showing the usefulness of the method for arsenic contained in environmental compartments. We also describe the apparent effect of the arsenic valence state on ICP-OES detection for total arsenic.

AB - To monitor the oxidation of arsenite to arsenate in oxidizing and bioleaching reactors, speciation analysis of the inorganic arsenic compounds is required. Existing arsenic speciation analysis techniques are based on the use of liquid chromatography columns coupled to detector equipment such as inductively coupled plasma optical emission spectrometry (ICP-OES). These techniques are fairly complex and expensive. Therefore, for analysis of arsenic speciation in our studies on thermophilic iron and arsenic oxidation, we developed a simple (and inexpensive) method based on anionic ion exclusion chromatography using sulfuric acid as mobile phase, followed by UV detection. The detection limit was 0.1¿mg¿L-1 of arsenite (As3+) and 0.1¿mg¿L-1 of arsenate (As5+). The method was validated with samples from an iron-oxidizing bioreactor containing 0.12¿mol¿L-1 H2SO4, 1¿g¿L-1 arsenic, and 3¿g¿L-1 iron. Samples from arsenopyritic gold concentrates by partial bio-oxidation and leaching samples were analyzed, showing the usefulness of the method for arsenic contained in environmental compartments. We also describe the apparent effect of the arsenic valence state on ICP-OES detection for total arsenic.

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