Hox gene expression profiles during embryonic development of common sole

Menelaos Kavouras*, Emmanouil E. Malandrakis, Eleni Golomazou, Ioannis Konstantinidis, Ewout Blom, Arjan P. Palstra, Konstantinos Anastassiadis, Panagiota Panagiotaki, Athanasios Exadactylos

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Common sole (Solea solea) aquaculture production is based mostly on wild-caught breeders. Recently, the successful reproduction of first-generation fish that were reared in captivity was accomplished. A consistent good quality and quantity of produced eggs throughout the year, and of next-generationbroodstock, is important for reducing the overall cost of production. Hox genes play a pivotal role in normal embryonic development and alterations of their temporal expression level may be important for egg viability. Expression profile analysis of five hox genes (hoxa1a, hoxa2a, hoxa2b, hoxb1a and hoxb1b) involved in early embryonic development and of hoxa13a, which is involved in late stages, was carried out. Results revealed a premature and/or maternal expression of hoxa13a in sole embryos,and the detection of hoxa2a and hoxa2b genes as members of paralog group 2. Principal Component Analysis of hox gene expression in 54 ± 6 hours post fertilization embryos coming from wild-caught broodstock and a first-generation one reared in the hatchery, unveiled that these broodstocks are clearly distinct. In addition, their pairwise comparison revealed significant differences in the expression levels of hoxb1a and hoxb1b genes. Hox gene regulation during embryonic development could give valuable insight into rearing sole broodstocks with different origin in concert, and also into gaining a steady mass production of eggs, either in quality or quantity, all year round.
Original languageEnglish
Number of pages16
JournalAnimal Biology
Volume69
Issue number2
Early online date18 Jul 2018
DOIs
Publication statusPublished - 25 Apr 2019

Fingerprint

gene expression
breeding stock
embryonic development
gene
embryogenesis
egg
genes
embryo
embryo (animal)
aquaculture production
Solea solea
captivity
egg quality
fertilization (reproduction)
hatchery
production costs
hatcheries
rearing
aquaculture
principal component analysis

Keywords

  • Broodstock
  • egg quality
  • homeobox genes
  • reproduction
  • Solea solea

Cite this

Kavouras, M., Malandrakis, E. E., Golomazou, E., Konstantinidis, I., Blom, E., Palstra, A. P., ... Exadactylos, A. (2019). Hox gene expression profiles during embryonic development of common sole. Animal Biology, 69(2). https://doi.org/10.1163/15707563-17000123
Kavouras, Menelaos ; Malandrakis, Emmanouil E. ; Golomazou, Eleni ; Konstantinidis, Ioannis ; Blom, Ewout ; Palstra, Arjan P. ; Anastassiadis, Konstantinos ; Panagiotaki, Panagiota ; Exadactylos, Athanasios. / Hox gene expression profiles during embryonic development of common sole. In: Animal Biology. 2019 ; Vol. 69, No. 2.
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abstract = "Common sole (Solea solea) aquaculture production is based mostly on wild-caught breeders. Recently, the successful reproduction of first-generation fish that were reared in captivity was accomplished. A consistent good quality and quantity of produced eggs throughout the year, and of next-generationbroodstock, is important for reducing the overall cost of production. Hox genes play a pivotal role in normal embryonic development and alterations of their temporal expression level may be important for egg viability. Expression profile analysis of five hox genes (hoxa1a, hoxa2a, hoxa2b, hoxb1a and hoxb1b) involved in early embryonic development and of hoxa13a, which is involved in late stages, was carried out. Results revealed a premature and/or maternal expression of hoxa13a in sole embryos,and the detection of hoxa2a and hoxa2b genes as members of paralog group 2. Principal Component Analysis of hox gene expression in 54 ± 6 hours post fertilization embryos coming from wild-caught broodstock and a first-generation one reared in the hatchery, unveiled that these broodstocks are clearly distinct. In addition, their pairwise comparison revealed significant differences in the expression levels of hoxb1a and hoxb1b genes. Hox gene regulation during embryonic development could give valuable insight into rearing sole broodstocks with different origin in concert, and also into gaining a steady mass production of eggs, either in quality or quantity, all year round.",
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Kavouras, M, Malandrakis, EE, Golomazou, E, Konstantinidis, I, Blom, E, Palstra, AP, Anastassiadis, K, Panagiotaki, P & Exadactylos, A 2019, 'Hox gene expression profiles during embryonic development of common sole', Animal Biology, vol. 69, no. 2. https://doi.org/10.1163/15707563-17000123

Hox gene expression profiles during embryonic development of common sole. / Kavouras, Menelaos; Malandrakis, Emmanouil E.; Golomazou, Eleni; Konstantinidis, Ioannis; Blom, Ewout; Palstra, Arjan P.; Anastassiadis, Konstantinos; Panagiotaki, Panagiota; Exadactylos, Athanasios.

In: Animal Biology, Vol. 69, No. 2, 25.04.2019.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Hox gene expression profiles during embryonic development of common sole

AU - Kavouras, Menelaos

AU - Malandrakis, Emmanouil E.

AU - Golomazou, Eleni

AU - Konstantinidis, Ioannis

AU - Blom, Ewout

AU - Palstra, Arjan P.

AU - Anastassiadis, Konstantinos

AU - Panagiotaki, Panagiota

AU - Exadactylos, Athanasios

PY - 2019/4/25

Y1 - 2019/4/25

N2 - Common sole (Solea solea) aquaculture production is based mostly on wild-caught breeders. Recently, the successful reproduction of first-generation fish that were reared in captivity was accomplished. A consistent good quality and quantity of produced eggs throughout the year, and of next-generationbroodstock, is important for reducing the overall cost of production. Hox genes play a pivotal role in normal embryonic development and alterations of their temporal expression level may be important for egg viability. Expression profile analysis of five hox genes (hoxa1a, hoxa2a, hoxa2b, hoxb1a and hoxb1b) involved in early embryonic development and of hoxa13a, which is involved in late stages, was carried out. Results revealed a premature and/or maternal expression of hoxa13a in sole embryos,and the detection of hoxa2a and hoxa2b genes as members of paralog group 2. Principal Component Analysis of hox gene expression in 54 ± 6 hours post fertilization embryos coming from wild-caught broodstock and a first-generation one reared in the hatchery, unveiled that these broodstocks are clearly distinct. In addition, their pairwise comparison revealed significant differences in the expression levels of hoxb1a and hoxb1b genes. Hox gene regulation during embryonic development could give valuable insight into rearing sole broodstocks with different origin in concert, and also into gaining a steady mass production of eggs, either in quality or quantity, all year round.

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KW - Broodstock

KW - egg quality

KW - homeobox genes

KW - reproduction

KW - Solea solea

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