How pea fractions with different protein composition and purity can substitute WPI in heat-set gels

Remco Kornet, Simone Penris, Paul Venema*, Atze Jan van der Goot, Marcel B.J. Meinders, Erik van der Linden

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)


In this study we explored the gelling behaviour of a pea protein concentrate (PPC), an albumin-fraction (ALB-F) and a globulin-rich fraction (GLB-RF), in comparison with and as substitute for whey protein isolate (WPI), by small oscillatory and large amplitude oscillatory shear (SAOS and LAOS) rheology. It was found that PPC formed the firmest gels (defined as highest elastic modulus), but this gel was not as firm as a pure WPI gel. The ALB-F formed the weakest gel due to its low protein purity. For a better view on the albumin gelling behaviour, ALB-F was further diafiltrated and the albumin-enriched fraction was labelled ALB-RF. It turned out that albumins formed firmer gels per mass unit of protein than globulins. Also, the energy dissipation ratios – a measure for the plasticity of the gel – were determined as a function of strain. The ALB-RF gel showed an increase in plastic response at larger strains compared to the GLB-RF gel (40% and 10% strain, respectively). ALB-F, PPC and GLB-RF were also examined on their ability to substitute WPI in heat-set gels. It was found that ALB-F/WPI mixtures formed firm gels and were least sensitive to changes in pH and ionic strength. It also appeared that disulphide bonding plays a more important role in the ALB-F/WPI mixtures upon heat-set gelation compared to the PPC/WPI and GLB-RF/WPI mixtures. The use of pea fractions as a substitute for WPI, particularly the ALB-F, could improve the resource efficiency of pea as an ingredient source.

Original languageEnglish
Article number106891
JournalFood Hydrocolloids
Publication statusPublished - Nov 2021


  • Heat-set gelation
  • Mild fractionation
  • Plant protein
  • Whey protein isolate
  • Yellow pea


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