How Does Alkali Aid Protein Extraction in Green Tea Leaf Residue: A Basis for Integrated Biorefinery of Leaves

C. Zhang, J.P.M. Sanders, T.T. Xiao, M.E. Bruins

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

Leaf protein can be obtained cost-efficiently by alkaline extraction, but overuse of chemicals and low quality of (denatured) protein limits its application. The research objective was to investigate how alkali aids protein extraction of green tea leaf residue, and use these results for further improvements in alkaline protein biorefinery. Protein extraction yield was studied for correlation to morphology of leaf tissue structure, protein solubility and hydrolysis degree, and yields of non-protein components obtained at various conditions. Alkaline protein extraction was not facilitated by increased solubility or hydrolysis of protein, but positively correlated to leaf tissue disruption. HG pectin, RGII pectin, and organic acids were extracted before protein extraction, which was followed by the extraction of cellulose and hemi-cellulose. RGI pectin and lignin were both linear to protein yield. The yields of these two components were 80% and 25% respectively when 95% protein was extracted, which indicated that RGI pectin is more likely to be the key limitation to leaf protein extraction. An integrated biorefinery was designed based on these results. Introduction
Original languageEnglish
Article numbere0133046
JournalPLoS ONE
Volume10
Issue number7
DOIs
Publication statusPublished - 2015

Keywords

  • functional-properties
  • antioxidant activity
  • sugar-beet
  • cell-walls
  • cellulose
  • pectins
  • biomass
  • acid
  • degradation
  • hydrolysis

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