How cell wall complexity influences saccharification efficiency in Miscanthus sinensis

Amanda P. De Souza, Claire Lessa Alvim Kamei, Andres Francisco Torres Salvador, Sivakumar Pattathil, Michael G. Hahn, Luisa M. Trindade, Marcos S. Buckeridge*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

40 Citations (Scopus)

Abstract

The production of bioenergy from grasses has been developing quickly during the last decade, with Miscanthus being among the most important choices for production of bioethanol. However, one of the key barriers to producing bioethanol is the lack of information about cell wall structure. Cell walls are thought to display compositional differences that lead to emergence of a very high level of complexity, resulting in great diversity in cell wall architectures. In this work, a set of different techniques was used to access the complexity of cell walls of different genotypes of Miscanthus sinensis in order to understand how they interfere with saccharification efficiency. Three genotypes of M. sinensis displaying different patterns of correlation between lignin content and saccharification efficiency were subjected to cell wall analysis by quantitative/qualitative analytical techniques such as monosaccharide composition, oligosaccharide profiling, and glycome profiling. When saccharification efficiency was correlated negatively with lignin, the structural features of arabinoxylan and xyloglucan were found to contribute positively to hydrolysis. In the absence of such correlation, different types of pectins, and some mannans contributed to saccharification efficiency. Different genotypes of M. sinensis were shown to display distinct interactions among their cell wall components, which seem to influence cell wall hydrolysis.

Original languageEnglish
Pages (from-to)4351-4365
JournalJournal of Experimental Botany
Volume66
Issue number14
DOIs
Publication statusPublished - 2015

Fingerprint

Miscanthus sinensis
saccharification
Cell Wall
cell walls
Lignin
Genotype
genotype
lignin
hydrolysis
mannans
Hydrolysis
bioethanol
Miscanthus
Pectins
xyloglucans
arabinoxylan
Mannans
cell wall components
ethanol production
monosaccharides

Keywords

  • Bioenergy
  • Cell wall polysaccharides
  • Lignin
  • Recalcitrance
  • Saccharification

Cite this

De Souza, A. P., Lessa Alvim Kamei, C., Torres Salvador, A. F., Pattathil, S., Hahn, M. G., Trindade, L. M., & Buckeridge, M. S. (2015). How cell wall complexity influences saccharification efficiency in Miscanthus sinensis. Journal of Experimental Botany, 66(14), 4351-4365. https://doi.org/10.1093/jxb/erv183
De Souza, Amanda P. ; Lessa Alvim Kamei, Claire ; Torres Salvador, Andres Francisco ; Pattathil, Sivakumar ; Hahn, Michael G. ; Trindade, Luisa M. ; Buckeridge, Marcos S. / How cell wall complexity influences saccharification efficiency in Miscanthus sinensis. In: Journal of Experimental Botany. 2015 ; Vol. 66, No. 14. pp. 4351-4365.
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De Souza, AP, Lessa Alvim Kamei, C, Torres Salvador, AF, Pattathil, S, Hahn, MG, Trindade, LM & Buckeridge, MS 2015, 'How cell wall complexity influences saccharification efficiency in Miscanthus sinensis', Journal of Experimental Botany, vol. 66, no. 14, pp. 4351-4365. https://doi.org/10.1093/jxb/erv183

How cell wall complexity influences saccharification efficiency in Miscanthus sinensis. / De Souza, Amanda P.; Lessa Alvim Kamei, Claire; Torres Salvador, Andres Francisco; Pattathil, Sivakumar; Hahn, Michael G.; Trindade, Luisa M.; Buckeridge, Marcos S.

In: Journal of Experimental Botany, Vol. 66, No. 14, 2015, p. 4351-4365.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - De Souza, Amanda P.

AU - Lessa Alvim Kamei, Claire

AU - Torres Salvador, Andres Francisco

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AU - Hahn, Michael G.

AU - Trindade, Luisa M.

AU - Buckeridge, Marcos S.

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AB - The production of bioenergy from grasses has been developing quickly during the last decade, with Miscanthus being among the most important choices for production of bioethanol. However, one of the key barriers to producing bioethanol is the lack of information about cell wall structure. Cell walls are thought to display compositional differences that lead to emergence of a very high level of complexity, resulting in great diversity in cell wall architectures. In this work, a set of different techniques was used to access the complexity of cell walls of different genotypes of Miscanthus sinensis in order to understand how they interfere with saccharification efficiency. Three genotypes of M. sinensis displaying different patterns of correlation between lignin content and saccharification efficiency were subjected to cell wall analysis by quantitative/qualitative analytical techniques such as monosaccharide composition, oligosaccharide profiling, and glycome profiling. When saccharification efficiency was correlated negatively with lignin, the structural features of arabinoxylan and xyloglucan were found to contribute positively to hydrolysis. In the absence of such correlation, different types of pectins, and some mannans contributed to saccharification efficiency. Different genotypes of M. sinensis were shown to display distinct interactions among their cell wall components, which seem to influence cell wall hydrolysis.

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De Souza AP, Lessa Alvim Kamei C, Torres Salvador AF, Pattathil S, Hahn MG, Trindade LM et al. How cell wall complexity influences saccharification efficiency in Miscanthus sinensis. Journal of Experimental Botany. 2015;66(14):4351-4365. https://doi.org/10.1093/jxb/erv183