Host- and stage-dependent secretome of the arbuscular mycorrhizal fungus Rhizophagus irregularis

Tian Zeng, Rens Holmer, Jan Hontelez, Bas te Lintel-Hekkert, Lucky Marufu, Thijs de Zeeuw, Fangyuan Wu, Elio Schijlen, Ton Bisseling, Erik Limpens

Research output: Contribution to journalArticleAcademicpeer-review

20 Citations (Scopus)

Abstract

Arbuscular mycorrhizal fungi form the most wide‐spread endosymbiosis with plants. There is very little host‐specificity in this interaction, however host preferences as well as varying symbiotic efficiencies have been observed. We hypothesize that secreted proteins (SPs) may act as fungal effectors to control symbiotic efficiency in a host‐dependent manner. Therefore, we studied whether AM fungi adjust their secretome in a host‐ and stage‐dependent manner to contribute to their extremely wide host‐range. We investigated the expression of SP‐encoding genes of Rhizophagus irregularis in three evolutionary distantly‐related plant species, Medicago truncatula, Nicotiana benthamiana and Allium schoenoprasum. In addition we used laser microdissection in combination with RNAseq to study SP expression at different stages of the interaction in Medicago. Our data indicate that most expressed SPs show roughly equal expression levels in the interaction with all three host plants. In addition, a subset shows significant differential expression depending on the host plant. Furthermore, SP expression is controlled locally in the hyphal network in response to host dependent cues. Overall, this study presents a comprehensive analysis of the R. irregularis secretome, which now offers a solid basis to direct functional studies on the role of fungal SPs in AM symbiosis.
Original languageEnglish
Pages (from-to)411-425
JournalThe Plant Journal
Volume94
Issue number3
DOIs
Publication statusPublished - May 2018

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mycorrhizal fungi
Fungi
symbiosis
Symbiosis
host plants
Allium schoenoprasum
protein synthesis
Chive
Proteins
Medicago
proteins
Nicotiana benthamiana
Medicago truncatula
host preferences
Efficiency
Microdissection
Fungal Proteins
lasers
Tobacco
Cues

Cite this

@article{c645fd4324fb4a63a2163b988ed1c475,
title = "Host- and stage-dependent secretome of the arbuscular mycorrhizal fungus Rhizophagus irregularis",
abstract = "Arbuscular mycorrhizal fungi form the most wide‐spread endosymbiosis with plants. There is very little host‐specificity in this interaction, however host preferences as well as varying symbiotic efficiencies have been observed. We hypothesize that secreted proteins (SPs) may act as fungal effectors to control symbiotic efficiency in a host‐dependent manner. Therefore, we studied whether AM fungi adjust their secretome in a host‐ and stage‐dependent manner to contribute to their extremely wide host‐range. We investigated the expression of SP‐encoding genes of Rhizophagus irregularis in three evolutionary distantly‐related plant species, Medicago truncatula, Nicotiana benthamiana and Allium schoenoprasum. In addition we used laser microdissection in combination with RNAseq to study SP expression at different stages of the interaction in Medicago. Our data indicate that most expressed SPs show roughly equal expression levels in the interaction with all three host plants. In addition, a subset shows significant differential expression depending on the host plant. Furthermore, SP expression is controlled locally in the hyphal network in response to host dependent cues. Overall, this study presents a comprehensive analysis of the R. irregularis secretome, which now offers a solid basis to direct functional studies on the role of fungal SPs in AM symbiosis.",
author = "Tian Zeng and Rens Holmer and Jan Hontelez and {te Lintel-Hekkert}, Bas and Lucky Marufu and {de Zeeuw}, Thijs and Fangyuan Wu and Elio Schijlen and Ton Bisseling and Erik Limpens",
year = "2018",
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language = "English",
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Host- and stage-dependent secretome of the arbuscular mycorrhizal fungus Rhizophagus irregularis. / Zeng, Tian; Holmer, Rens; Hontelez, Jan; te Lintel-Hekkert, Bas; Marufu, Lucky; de Zeeuw, Thijs; Wu, Fangyuan; Schijlen, Elio; Bisseling, Ton; Limpens, Erik.

In: The Plant Journal, Vol. 94, No. 3, 05.2018, p. 411-425.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Host- and stage-dependent secretome of the arbuscular mycorrhizal fungus Rhizophagus irregularis

AU - Zeng, Tian

AU - Holmer, Rens

AU - Hontelez, Jan

AU - te Lintel-Hekkert, Bas

AU - Marufu, Lucky

AU - de Zeeuw, Thijs

AU - Wu, Fangyuan

AU - Schijlen, Elio

AU - Bisseling, Ton

AU - Limpens, Erik

PY - 2018/5

Y1 - 2018/5

N2 - Arbuscular mycorrhizal fungi form the most wide‐spread endosymbiosis with plants. There is very little host‐specificity in this interaction, however host preferences as well as varying symbiotic efficiencies have been observed. We hypothesize that secreted proteins (SPs) may act as fungal effectors to control symbiotic efficiency in a host‐dependent manner. Therefore, we studied whether AM fungi adjust their secretome in a host‐ and stage‐dependent manner to contribute to their extremely wide host‐range. We investigated the expression of SP‐encoding genes of Rhizophagus irregularis in three evolutionary distantly‐related plant species, Medicago truncatula, Nicotiana benthamiana and Allium schoenoprasum. In addition we used laser microdissection in combination with RNAseq to study SP expression at different stages of the interaction in Medicago. Our data indicate that most expressed SPs show roughly equal expression levels in the interaction with all three host plants. In addition, a subset shows significant differential expression depending on the host plant. Furthermore, SP expression is controlled locally in the hyphal network in response to host dependent cues. Overall, this study presents a comprehensive analysis of the R. irregularis secretome, which now offers a solid basis to direct functional studies on the role of fungal SPs in AM symbiosis.

AB - Arbuscular mycorrhizal fungi form the most wide‐spread endosymbiosis with plants. There is very little host‐specificity in this interaction, however host preferences as well as varying symbiotic efficiencies have been observed. We hypothesize that secreted proteins (SPs) may act as fungal effectors to control symbiotic efficiency in a host‐dependent manner. Therefore, we studied whether AM fungi adjust their secretome in a host‐ and stage‐dependent manner to contribute to their extremely wide host‐range. We investigated the expression of SP‐encoding genes of Rhizophagus irregularis in three evolutionary distantly‐related plant species, Medicago truncatula, Nicotiana benthamiana and Allium schoenoprasum. In addition we used laser microdissection in combination with RNAseq to study SP expression at different stages of the interaction in Medicago. Our data indicate that most expressed SPs show roughly equal expression levels in the interaction with all three host plants. In addition, a subset shows significant differential expression depending on the host plant. Furthermore, SP expression is controlled locally in the hyphal network in response to host dependent cues. Overall, this study presents a comprehensive analysis of the R. irregularis secretome, which now offers a solid basis to direct functional studies on the role of fungal SPs in AM symbiosis.

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