Hijacking CRISPR-Cas for high-throughput bacterial metabolic engineering

advances and prospects

Research output: Contribution to journalArticleAcademicpeer-review

21 Citations (Scopus)

Abstract

High engineering efficiencies are required for industrial strain development. Due to its user-friendliness and its stringency, CRISPR-Cas-based technologies have strongly increased genome engineering efficiencies in bacteria. This has enabled more rapid metabolic engineering of both the model host Escherichia coli and non-model organisms like Clostridia, Bacilli, Streptomycetes and cyanobacteria, opening new possibilities to use these organisms as improved cell factories. The discovery of novel Cas9-like systems from diverse microbial environments will extend the repertoire of applications and broaden the range of organisms in which it can be used to create novel production hosts. This review analyses the current status of prokaryotic metabolic engineering towards the production of biotechnologically relevant products, based on the exploitation of different CRISPR-related DNA/RNA endonuclease variants.
Original languageEnglish
Pages (from-to)146-157
JournalCurrent Opinion in Biotechnology
Volume50
DOIs
Publication statusPublished - 1 Apr 2018

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Clustered Regularly Interspaced Short Palindromic Repeats
Metabolic engineering
Metabolic Engineering
Throughput
Clostridium
Endonucleases
Deoxyribonuclease I
Cyanobacteria
Bacilli
RNA
Escherichia coli
Bacillus
Industrial plants
Bacteria
DNA
Genes
Genome
Technology

Cite this

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title = "Hijacking CRISPR-Cas for high-throughput bacterial metabolic engineering: advances and prospects",
abstract = "High engineering efficiencies are required for industrial strain development. Due to its user-friendliness and its stringency, CRISPR-Cas-based technologies have strongly increased genome engineering efficiencies in bacteria. This has enabled more rapid metabolic engineering of both the model host Escherichia coli and non-model organisms like Clostridia, Bacilli, Streptomycetes and cyanobacteria, opening new possibilities to use these organisms as improved cell factories. The discovery of novel Cas9-like systems from diverse microbial environments will extend the repertoire of applications and broaden the range of organisms in which it can be used to create novel production hosts. This review analyses the current status of prokaryotic metabolic engineering towards the production of biotechnologically relevant products, based on the exploitation of different CRISPR-related DNA/RNA endonuclease variants.",
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Hijacking CRISPR-Cas for high-throughput bacterial metabolic engineering : advances and prospects. / Mougiakos, Ioannis; Bosma, Elleke F.; Ganguly, Joyshree; van der Oost, John; van Kranenburg, Richard.

In: Current Opinion in Biotechnology, Vol. 50, 01.04.2018, p. 146-157.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Mougiakos, Ioannis

AU - Bosma, Elleke F.

AU - Ganguly, Joyshree

AU - van der Oost, John

AU - van Kranenburg, Richard

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