Highly efficient mono-functionalization of knob-in-hole antibodies with strain-promoted click chemistry

Jorick J. Bruins, Criss van de Wouw, Wagner Koen, Bartels Lina, Bauke Albada, F.L. van Delft

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Knob-in-hole antibodies can be utilized to introduce a single tag for chemo-enzymatic functionalization. By either introducing a single C-terminal sortase tag (sortase-tag expressed protein ligation) or tyrosine tag (G4Y), mono-functionalization of the monoclonal antibody trastuzumab was achieved rapidly and in high yields. This method was applied to selectively and efficiently introduce a single fluorescent tag, cytokine or single-chain variable fragment, as well as produce clean homo dimers of trastuzumab.

Original languageEnglish
Article number4
Pages (from-to)11801-11807
JournalJournal of the American Chemical Society
Volume4
DOIs
Publication statusPublished - 8 Jul 2019

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Click Chemistry
Knobs
Monoclonal antibodies
Antibodies
Dimers
Proteins
Single-Chain Antibodies
Ligation
Tyrosine
Monoclonal Antibodies
Cytokines
Trastuzumab
sortase C

Cite this

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title = "Highly efficient mono-functionalization of knob-in-hole antibodies with strain-promoted click chemistry",
abstract = "Knob-in-hole antibodies can be utilized to introduce a single tag for chemo-enzymatic functionalization. By either introducing a single C-terminal sortase tag (sortase-tag expressed protein ligation) or tyrosine tag (G4Y), mono-functionalization of the monoclonal antibody trastuzumab was achieved rapidly and in high yields. This method was applied to selectively and efficiently introduce a single fluorescent tag, cytokine or single-chain variable fragment, as well as produce clean homo dimers of trastuzumab.",
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Highly efficient mono-functionalization of knob-in-hole antibodies with strain-promoted click chemistry. / Bruins, Jorick J.; van de Wouw, Criss; Koen, Wagner; Lina, Bartels; Albada, Bauke; van Delft, F.L.

In: Journal of the American Chemical Society, Vol. 4, 4, 08.07.2019, p. 11801-11807.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Bruins, Jorick J.

AU - van de Wouw, Criss

AU - Koen, Wagner

AU - Lina, Bartels

AU - Albada, Bauke

AU - van Delft, F.L.

PY - 2019/7/8

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AB - Knob-in-hole antibodies can be utilized to introduce a single tag for chemo-enzymatic functionalization. By either introducing a single C-terminal sortase tag (sortase-tag expressed protein ligation) or tyrosine tag (G4Y), mono-functionalization of the monoclonal antibody trastuzumab was achieved rapidly and in high yields. This method was applied to selectively and efficiently introduce a single fluorescent tag, cytokine or single-chain variable fragment, as well as produce clean homo dimers of trastuzumab.

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DO - 10.1021/acsomega.9b01727

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JF - Journal of the American Chemical Society

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