Highly efficient mono-functionalization of knob-in-hole antibodies with strain-promoted click chemistry

Jorick J. Bruins, Criss van de Wouw, Wagner Koen, Bartels Lina, Bauke Albada*, F.L. van Delft

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Knob-in-hole antibodies can be utilized to introduce a single tag for chemo-enzymatic functionalization. By either introducing a single C-terminal sortase tag (sortase-tag expressed protein ligation) or tyrosine tag (G4Y), mono-functionalization of the monoclonal antibody trastuzumab was achieved rapidly and in high yields. This method was applied to selectively and efficiently introduce a single fluorescent tag, cytokine or single-chain variable fragment, as well as produce clean homo dimers of trastuzumab.

Original languageEnglish
Article number4
Pages (from-to)11801-11807
JournalJournal of the American Chemical Society
Volume4
DOIs
Publication statusPublished - 8 Jul 2019

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