Higher Chain Length Distribution in Debranched Type-3 Resistant Starches (RS3) Increases TLR Signaling and Supports Dendritic Cell Cytokine Production

Alexia F.P. Lépine*, Roderick H.J. de Hilster, Hans Leemhuis, Lizette Oudhuis, Piet L. Buwalda, Paul de Vos

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Scope: Resistant starches (RSs) are classically considered to elicit health benefits through fermentation. However, it is recently shown that RSs can also support health by direct immune interactions. Therefore, it has been hypothesized that the structural traits of RSs might impact the health benefits associated with their consumption. Methods and results: Effects of crystallinity, molecular weight, and chain length distribution of RSs are determined on immune Toll-like receptors (TLRs), dendritic cells (DCs), and T-cell cytokines production. To this end, four type-3 RSs (RS3) are compared, namely Paselli WFR, JD150, debranched Etenia, and Amylose fraction V, which are extracted from potatoes and enzymatically modified. Dextrose equivalent seems to be the most important feature influencing immune signaling via activation of TLRs. TLR2 and TLR4 are most strongly stimulated. Especially Paselli WFR is a potent activator of multiple receptors. Moreover, the presence of amylose, even to residual levels, enhances DC and T-cell cytokine responses. Paselli WFR and Amylose fraction V influence T-cell polarization. Conclusions: It has been shown here that chain length and particularly dextrose equivalent are critical features for immune activation. This knowledge might lead to tailoring and design of immune-active RS formulations.

Original languageEnglish
Article number1801007
JournalMolecular Nutrition and Food Research
Volume63
Issue number2
Early online date9 Nov 2018
DOIs
Publication statusPublished - Jan 2019

Fingerprint

resistant starch
Toll-Like Receptors
dendritic cells
Starch
Dendritic Cells
cytokines
Cytokines
Amylose
amylose
T-lymphocytes
Insurance Benefits
T-Lymphocytes
Glucose
glucose
Solanum tuberosum
Fermentation
Toll-like receptors
Molecular Weight
fermentation
potatoes

Keywords

  • Caco-2
  • dendritic cells
  • resistant starches
  • T-cells
  • Toll-like receptors

Cite this

@article{bb0b5aa09aaf4589bc08f595d80684b5,
title = "Higher Chain Length Distribution in Debranched Type-3 Resistant Starches (RS3) Increases TLR Signaling and Supports Dendritic Cell Cytokine Production",
abstract = "Scope: Resistant starches (RSs) are classically considered to elicit health benefits through fermentation. However, it is recently shown that RSs can also support health by direct immune interactions. Therefore, it has been hypothesized that the structural traits of RSs might impact the health benefits associated with their consumption. Methods and results: Effects of crystallinity, molecular weight, and chain length distribution of RSs are determined on immune Toll-like receptors (TLRs), dendritic cells (DCs), and T-cell cytokines production. To this end, four type-3 RSs (RS3) are compared, namely Paselli WFR, JD150, debranched Etenia, and Amylose fraction V, which are extracted from potatoes and enzymatically modified. Dextrose equivalent seems to be the most important feature influencing immune signaling via activation of TLRs. TLR2 and TLR4 are most strongly stimulated. Especially Paselli WFR is a potent activator of multiple receptors. Moreover, the presence of amylose, even to residual levels, enhances DC and T-cell cytokine responses. Paselli WFR and Amylose fraction V influence T-cell polarization. Conclusions: It has been shown here that chain length and particularly dextrose equivalent are critical features for immune activation. This knowledge might lead to tailoring and design of immune-active RS formulations.",
keywords = "Caco-2, dendritic cells, resistant starches, T-cells, Toll-like receptors",
author = "L{\'e}pine, {Alexia F.P.} and {de Hilster}, {Roderick H.J.} and Hans Leemhuis and Lizette Oudhuis and Buwalda, {Piet L.} and {de Vos}, Paul",
year = "2019",
month = "1",
doi = "10.1002/mnfr.201801007",
language = "English",
volume = "63",
journal = "Molecular Nutrition & Food Research",
issn = "1613-4125",
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}

Higher Chain Length Distribution in Debranched Type-3 Resistant Starches (RS3) Increases TLR Signaling and Supports Dendritic Cell Cytokine Production. / Lépine, Alexia F.P.; de Hilster, Roderick H.J.; Leemhuis, Hans; Oudhuis, Lizette; Buwalda, Piet L.; de Vos, Paul.

In: Molecular Nutrition and Food Research, Vol. 63, No. 2, 1801007, 01.2019.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Higher Chain Length Distribution in Debranched Type-3 Resistant Starches (RS3) Increases TLR Signaling and Supports Dendritic Cell Cytokine Production

AU - Lépine, Alexia F.P.

AU - de Hilster, Roderick H.J.

AU - Leemhuis, Hans

AU - Oudhuis, Lizette

AU - Buwalda, Piet L.

AU - de Vos, Paul

PY - 2019/1

Y1 - 2019/1

N2 - Scope: Resistant starches (RSs) are classically considered to elicit health benefits through fermentation. However, it is recently shown that RSs can also support health by direct immune interactions. Therefore, it has been hypothesized that the structural traits of RSs might impact the health benefits associated with their consumption. Methods and results: Effects of crystallinity, molecular weight, and chain length distribution of RSs are determined on immune Toll-like receptors (TLRs), dendritic cells (DCs), and T-cell cytokines production. To this end, four type-3 RSs (RS3) are compared, namely Paselli WFR, JD150, debranched Etenia, and Amylose fraction V, which are extracted from potatoes and enzymatically modified. Dextrose equivalent seems to be the most important feature influencing immune signaling via activation of TLRs. TLR2 and TLR4 are most strongly stimulated. Especially Paselli WFR is a potent activator of multiple receptors. Moreover, the presence of amylose, even to residual levels, enhances DC and T-cell cytokine responses. Paselli WFR and Amylose fraction V influence T-cell polarization. Conclusions: It has been shown here that chain length and particularly dextrose equivalent are critical features for immune activation. This knowledge might lead to tailoring and design of immune-active RS formulations.

AB - Scope: Resistant starches (RSs) are classically considered to elicit health benefits through fermentation. However, it is recently shown that RSs can also support health by direct immune interactions. Therefore, it has been hypothesized that the structural traits of RSs might impact the health benefits associated with their consumption. Methods and results: Effects of crystallinity, molecular weight, and chain length distribution of RSs are determined on immune Toll-like receptors (TLRs), dendritic cells (DCs), and T-cell cytokines production. To this end, four type-3 RSs (RS3) are compared, namely Paselli WFR, JD150, debranched Etenia, and Amylose fraction V, which are extracted from potatoes and enzymatically modified. Dextrose equivalent seems to be the most important feature influencing immune signaling via activation of TLRs. TLR2 and TLR4 are most strongly stimulated. Especially Paselli WFR is a potent activator of multiple receptors. Moreover, the presence of amylose, even to residual levels, enhances DC and T-cell cytokine responses. Paselli WFR and Amylose fraction V influence T-cell polarization. Conclusions: It has been shown here that chain length and particularly dextrose equivalent are critical features for immune activation. This knowledge might lead to tailoring and design of immune-active RS formulations.

KW - Caco-2

KW - dendritic cells

KW - resistant starches

KW - T-cells

KW - Toll-like receptors

U2 - 10.1002/mnfr.201801007

DO - 10.1002/mnfr.201801007

M3 - Article

VL - 63

JO - Molecular Nutrition & Food Research

JF - Molecular Nutrition & Food Research

SN - 1613-4125

IS - 2

M1 - 1801007

ER -