High-resolution separation of polychlorinated biphenyls by comprehensive two-dimensional gas chromatography

P. Korytar, P.E.G. Leonards, J. de Boer, U.A.Th. Brinkman

    Research output: Contribution to journalArticleAcademicpeer-review

    121 Citations (Scopus)

    Abstract

    Comprehensive two-dimensional gas chromatography (GCxGC) with micro electron-capture detection (ECD) has been optimised for the separation of polychlorinated biphenyl congeners with emphasis on the separation of 12 toxic non- and mono-ortho chlorinated biphenyls (CBs), viz. CBs 77, 81, 105, 114, 118, 123, 126, 156, 157, 167, 169 and 189. The selection of the first- and second-dimension columns and the temperature programme optimisation were carried out with a mixture of 90 CBs and the results are compared with those of one-dimensional GC. A complete separation of all 12 priority CBs was obtained with two column combinations, HP-1–HT-8 and HP-1–SupelcoWax-10. With the HP-1–HT-8 column set, ordered structures show up in the two-dimensional plane, with the number of chlorine substituents and their position (ortho vs. non-ortho) being the main parameters of interest. This can help with congener identification. Estimated detection limits are excellent, i.e. about 10 fg. To illustrate the potential and the versatility of GCxGC–ECD, a cod liver extract and a standard mixture of the 17 most toxic polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans together with 90 CBs were analysed as an application.
    Original languageEnglish
    Pages (from-to)203-213
    JournalJournal of Chromatography. A, Including electrophoresis and other separation methods
    Volume958
    DOIs
    Publication statusPublished - 2002

    Keywords

    • Gas chromatography
    • Polychlorinated biphenyls

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