High-Performance Liquid Chromatography–Mass Spectrometry Analysis of Plant Metabolites in Brassicaceae

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

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Abstract

The Brassicaceae family comprises a variety of plant species that are of high economic importance as -vegetables or industrial crops. This includes crops such as Brassica rapa (turnip, Bok Choi), B. oleracea (cabbages, broccoli, cauliflower, etc.), and B. napus (oil seed rape), and also includes the famous genetic model of plant research, Arabidopsis thaliana (thale cress). Brassicaceae plants contain a large variety of interesting secondary metabolites, including glucosinolates, hydroxycinnamic acids, and flavonoids. These metabolites are also of particular importance due to their proposed positive effects on human health. Next to these well-known groups of phytochemicals, many more metabolites are of course also present in crude extracts prepared from Brassica and Arabidopsis plant material.High-pressure liquid chromatography coupled to mass spectrometry (HPLC-MS), especially if combined with a high mass resolution instrument such as a QTOF MS, is a powerful approach to separate, detect, and annotate metabolites present in crude aqueous-alcohol plant extracts. Using an essentially unbiased procedure that takes into account all metabolite mass signals from the raw data files, detailed information on the relative abundance of hundreds of both known and, as yet, unknown semipolar metabolites can be obtained. These comprehensive metabolomics data can then be used to, for instance, identify genetic markers regulating metabolic composition, determine effects of (a)biotic stress or specific growth conditions, or establish metabolite changes occurring upon food processing or storage.This chapter describes in detail a procedure for preparing crude extracts and performing comprehensive HPLC-QTOF MS-based profiling of semi-polar metabolites in Brassicaceae plant material. Compounds present in the extract can be (partially or completely) annotated based on their accurate mass, their MS/MS fragments and on other specific chemical characteristics such as retention time and UV-absorbance spectrum.
Original languageEnglish
Title of host publicationPlant metabolomics : methods and protocols
EditorsN.G. Hardy, R.D. Hall
Pages111-128
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in molecular biology
PublisherSpringer – Humana Press
Number860

Fingerprint

Brassicaceae
spectroscopy
metabolites
liquids
Brassica napus
extracts
Arabidopsis thaliana
high performance liquid chromatography
industrial crops
Brassica rapa subsp. chinensis
food storage
turnips
cauliflower
coumaric acids
Brassica rapa
metabolomics
broccoli
vegetable crops
glucosinolates
Brassica oleracea

Cite this

de Vos, C. H., Schipper, A., & Hall, R. D. (2012). High-Performance Liquid Chromatography–Mass Spectrometry Analysis of Plant Metabolites in Brassicaceae. In N. G. Hardy, & R. D. Hall (Eds.), Plant metabolomics : methods and protocols (pp. 111-128). (Methods in molecular biology; No. 860). https://doi.org/10.1007/978-1-61779-594-7_8
de Vos, C.H. ; Schipper, A. ; Hall, R.D. / High-Performance Liquid Chromatography–Mass Spectrometry Analysis of Plant Metabolites in Brassicaceae. Plant metabolomics : methods and protocols. editor / N.G. Hardy ; R.D. Hall. 2012. pp. 111-128 (Methods in molecular biology; 860).
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de Vos, CH, Schipper, A & Hall, RD 2012, High-Performance Liquid Chromatography–Mass Spectrometry Analysis of Plant Metabolites in Brassicaceae. in NG Hardy & RD Hall (eds), Plant metabolomics : methods and protocols. Methods in molecular biology, no. 860, pp. 111-128. https://doi.org/10.1007/978-1-61779-594-7_8

High-Performance Liquid Chromatography–Mass Spectrometry Analysis of Plant Metabolites in Brassicaceae. / de Vos, C.H.; Schipper, A.; Hall, R.D.

Plant metabolomics : methods and protocols. ed. / N.G. Hardy; R.D. Hall. 2012. p. 111-128 (Methods in molecular biology; No. 860).

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

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N2 - The Brassicaceae family comprises a variety of plant species that are of high economic importance as -vegetables or industrial crops. This includes crops such as Brassica rapa (turnip, Bok Choi), B. oleracea (cabbages, broccoli, cauliflower, etc.), and B. napus (oil seed rape), and also includes the famous genetic model of plant research, Arabidopsis thaliana (thale cress). Brassicaceae plants contain a large variety of interesting secondary metabolites, including glucosinolates, hydroxycinnamic acids, and flavonoids. These metabolites are also of particular importance due to their proposed positive effects on human health. Next to these well-known groups of phytochemicals, many more metabolites are of course also present in crude extracts prepared from Brassica and Arabidopsis plant material.High-pressure liquid chromatography coupled to mass spectrometry (HPLC-MS), especially if combined with a high mass resolution instrument such as a QTOF MS, is a powerful approach to separate, detect, and annotate metabolites present in crude aqueous-alcohol plant extracts. Using an essentially unbiased procedure that takes into account all metabolite mass signals from the raw data files, detailed information on the relative abundance of hundreds of both known and, as yet, unknown semipolar metabolites can be obtained. These comprehensive metabolomics data can then be used to, for instance, identify genetic markers regulating metabolic composition, determine effects of (a)biotic stress or specific growth conditions, or establish metabolite changes occurring upon food processing or storage.This chapter describes in detail a procedure for preparing crude extracts and performing comprehensive HPLC-QTOF MS-based profiling of semi-polar metabolites in Brassicaceae plant material. Compounds present in the extract can be (partially or completely) annotated based on their accurate mass, their MS/MS fragments and on other specific chemical characteristics such as retention time and UV-absorbance spectrum.

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de Vos CH, Schipper A, Hall RD. High-Performance Liquid Chromatography–Mass Spectrometry Analysis of Plant Metabolites in Brassicaceae. In Hardy NG, Hall RD, editors, Plant metabolomics : methods and protocols. 2012. p. 111-128. (Methods in molecular biology; 860). https://doi.org/10.1007/978-1-61779-594-7_8