High-Level folate production in fermented foods by the B12 producer Lactobacillus reuteri JCM1112

F. dos Santos; A. Wegkamp; W.M. de Vos; E.J. Smid; J. Hugenholtz

doi:10.1128/AEM.02719-07

High-Level folate production in fermented foods by the B12 producer Lactobacillus reuteri JCM1112

F. dos Santos, A. Wegkamp, W.M. de Vos, E.J. Smid, J. Hugenholtz

Research output: Contribution to journal › Article › Academic › peer-review

117 Citations (Scopus)

Abstract

We observed that Lactobacillus reuteri JCM1112 produces B12 and folate. However, the folate/B12 mass ratio found was far below that desired for human consumption (170:1). We used metabolic engineering applying genetic and physiological approaches to improve this ratio and developed a generic and natural process that significantly increases folate production.

Original language	English
Pages (from-to)	3291-3294
Journal	Applied and Environmental Microbiology
Volume	74
Issue number	10
DOIs	https://doi.org/10.1128/AEM.02719-07
Publication status	Published - 2008

Keywords

lactic-acid bacteria
lactococcus-lactis
crl1098
biosynthesis
plantarum
cobalamin
sequence
systems

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10.1128/AEM.02719-07

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abstract = "We observed that Lactobacillus reuteri JCM1112 produces B12 and folate. However, the folate/B12 mass ratio found was far below that desired for human consumption (170:1). We used metabolic engineering applying genetic and physiological approaches to improve this ratio and developed a generic and natural process that significantly increases folate production.",

keywords = "lactic-acid bacteria, lactococcus-lactis, crl1098, biosynthesis, plantarum, cobalamin, sequence, systems",

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AU - de Vos, W.M.

AU - Smid, E.J.

AU - Hugenholtz, J.

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PY - 2008

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KW - lactic-acid bacteria

KW - lactococcus-lactis

KW - crl1098

KW - biosynthesis

KW - plantarum

KW - cobalamin

KW - sequence

KW - systems

U2 - 10.1128/AEM.02719-07

DO - 10.1128/AEM.02719-07

M3 - Article

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JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 10

ER -

High-Level folate production in fermented foods by the B12 producer Lactobacillus reuteri JCM1112

Abstract

Keywords

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