Hexadecylphosphocholine causes rapid cell death in canine mammary tumour cells

D. Duijsings, M. Houweling, A.B. Vaandrager, J.A. Mol, K.J. Teerds

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

Hexadecylphosphocholine (HePC, Miltefosine) is an antitumour phospholipid and known inducer of apoptosis in human breast cancer cells. The mechanism underlying the induction of cell death by HePC, however, is not clear yet. In this study, we have investigated the cytotoxic effects of HePC on canine mammary tumour cells (CMTs) in vitro. Upon addition of HePC, CMTs rapidly exhibited several features that resembled apoptotic cell death. Cells showed externalisation of phosphatidylserine, a hallmark of apoptosis, within 5 min after addition of HePC at concentrations as low as 10 µM. Furthermore, rapid swelling of mitochondria was observed. Rounding and detachment of cells followed within 30 min. However, fragmentation of nuclear DNA could not be observed. Overall, HePC was shown to induce a type of cell death in CMTs that in some aspects resembles apoptosis, though the process proceeds much more rapidly than reported for other tumour cell lines.
Original languageEnglish
Pages (from-to)185-193
JournalEuropean Journal of Pharmacology
Volume502
Issue number3
DOIs
Publication statusPublished - 2004

Keywords

  • phospholipid analog hexadecylphosphocholine
  • radiation-induced apoptosis
  • alkyl-lysophospholipids
  • ether lipids
  • inhibition
  • lines
  • ceramide
  • lysophosphatidylcholine
  • mitochondria
  • involvement

Fingerprint Dive into the research topics of 'Hexadecylphosphocholine causes rapid cell death in canine mammary tumour cells'. Together they form a unique fingerprint.

Cite this