In this chapter a method for the heterologous production of fungal proteins in the yeast Pichia pastoris is described. Starting with cloning of the sequence encoding the gene of interest into the expression vector, this protocol describes P. pastoris transformation, production of the protein in a fermentor, and purification of the protein. This method has successfully been used for the production of a number of fungal effector proteins.
|Title of host publication||Plant Fungal Pathogens: Methods and Protocols|
|Editors||M.D. Bolton, B.P.H.J. Thomma|
|Place of Publication||New York, USA|
|Publication status||Published - 2012|
|Name||Methods in Molecular Biology|
|Publisher||Humana Press, c/o Springer Science+Business Media|