Heterologous production of fungal effectors in Pichia pastoris

A. Kombrink

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

8 Citations (Scopus)


In this chapter a method for the heterologous production of fungal proteins in the yeast Pichia pastoris is described. Starting with cloning of the sequence encoding the gene of interest into the expression vector, this protocol describes P. pastoris transformation, production of the protein in a fermentor, and purification of the protein. This method has successfully been used for the production of a number of fungal effector proteins.
Original languageEnglish
Title of host publicationPlant Fungal Pathogens: Methods and Protocols
EditorsM.D. Bolton, B.P.H.J. Thomma
Place of PublicationNew York, USA
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
PublisherHumana Press, c/o Springer Science+Business Media


  • Fungal effector
  • Heterologous protein production
  • P. Pastoris
  • Yeast


Dive into the research topics of 'Heterologous production of fungal effectors in Pichia pastoris'. Together they form a unique fingerprint.

Cite this