Heterologous expression of two Arabidopsis starch dikinases in potato

Xuan Xu, Dianka Dees, Xing Feng Huang, Richard G.F. Visser, Luisa M. Trindade*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

Starch phosphate esters influence physiochemical properties of starch granules that are essential both for starch metabolism and industrial use of starches. To modify properties of potato starch and understand the effect of starch phosphorylation on starch metabolism in storage starch, the starch dikinases from Arabidopsis thaliana, glucan water dikinase 2 and 3 (AtGWD2 and AtGWD3), were heterologously expressed in potato tubers (Solanum tuberosum) from two genetic backgrounds: the amylose-containing clone Kardal and the amylose-free mutant amf. Modified starches showed altered granule morphology, but no significant changes in starch phosphate content were observed. Genes involved in starch metabolism did not show altered expression in the transgenic lines relatively to the control; however, sucrose synthase 4 (SUSY4) was upregulated in transgenic tubers with AtGWD2 expression. Taken together, these results demonstrated that expression of AtGWD2 and AtGWD3 are not suitable for engineering starch with a high phosphate content in potato tubers but can provide new insights in the process of starch phosphorylation in the amyloplast.
Original languageEnglish
Article number1600324
JournalStarch-Stärke
Volume70
Issue number1-2
Early online date2017
DOIs
Publication statusPublished - Jan 2018

Fingerprint

Solanum tuberosum
Arabidopsis
Starch
potatoes
starch
Metabolism
phosphates
amylose
Amylose
Phosphorylation
metabolism
Phosphates
phosphorylation
amyloplasts
modified starch
sucrose synthase
potato starch
glucans
starch granules
Plastids

Keywords

  • Glucan
  • Granule morphology
  • Starch metabolism
  • Storage starch
  • Transitory starch
  • Water dikinase

Cite this

@article{087a1ab53ebc4dcc931bf16eb2cf88c7,
title = "Heterologous expression of two Arabidopsis starch dikinases in potato",
abstract = "Starch phosphate esters influence physiochemical properties of starch granules that are essential both for starch metabolism and industrial use of starches. To modify properties of potato starch and understand the effect of starch phosphorylation on starch metabolism in storage starch, the starch dikinases from Arabidopsis thaliana, glucan water dikinase 2 and 3 (AtGWD2 and AtGWD3), were heterologously expressed in potato tubers (Solanum tuberosum) from two genetic backgrounds: the amylose-containing clone Kardal and the amylose-free mutant amf. Modified starches showed altered granule morphology, but no significant changes in starch phosphate content were observed. Genes involved in starch metabolism did not show altered expression in the transgenic lines relatively to the control; however, sucrose synthase 4 (SUSY4) was upregulated in transgenic tubers with AtGWD2 expression. Taken together, these results demonstrated that expression of AtGWD2 and AtGWD3 are not suitable for engineering starch with a high phosphate content in potato tubers but can provide new insights in the process of starch phosphorylation in the amyloplast.",
keywords = "Glucan, Granule morphology, Starch metabolism, Storage starch, Transitory starch, Water dikinase",
author = "Xuan Xu and Dianka Dees and Huang, {Xing Feng} and Visser, {Richard G.F.} and Trindade, {Luisa M.}",
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Heterologous expression of two Arabidopsis starch dikinases in potato. / Xu, Xuan; Dees, Dianka; Huang, Xing Feng; Visser, Richard G.F.; Trindade, Luisa M.

In: Starch-Stärke, Vol. 70, No. 1-2, 1600324 , 01.2018.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Heterologous expression of two Arabidopsis starch dikinases in potato

AU - Xu, Xuan

AU - Dees, Dianka

AU - Huang, Xing Feng

AU - Visser, Richard G.F.

AU - Trindade, Luisa M.

PY - 2018/1

Y1 - 2018/1

N2 - Starch phosphate esters influence physiochemical properties of starch granules that are essential both for starch metabolism and industrial use of starches. To modify properties of potato starch and understand the effect of starch phosphorylation on starch metabolism in storage starch, the starch dikinases from Arabidopsis thaliana, glucan water dikinase 2 and 3 (AtGWD2 and AtGWD3), were heterologously expressed in potato tubers (Solanum tuberosum) from two genetic backgrounds: the amylose-containing clone Kardal and the amylose-free mutant amf. Modified starches showed altered granule morphology, but no significant changes in starch phosphate content were observed. Genes involved in starch metabolism did not show altered expression in the transgenic lines relatively to the control; however, sucrose synthase 4 (SUSY4) was upregulated in transgenic tubers with AtGWD2 expression. Taken together, these results demonstrated that expression of AtGWD2 and AtGWD3 are not suitable for engineering starch with a high phosphate content in potato tubers but can provide new insights in the process of starch phosphorylation in the amyloplast.

AB - Starch phosphate esters influence physiochemical properties of starch granules that are essential both for starch metabolism and industrial use of starches. To modify properties of potato starch and understand the effect of starch phosphorylation on starch metabolism in storage starch, the starch dikinases from Arabidopsis thaliana, glucan water dikinase 2 and 3 (AtGWD2 and AtGWD3), were heterologously expressed in potato tubers (Solanum tuberosum) from two genetic backgrounds: the amylose-containing clone Kardal and the amylose-free mutant amf. Modified starches showed altered granule morphology, but no significant changes in starch phosphate content were observed. Genes involved in starch metabolism did not show altered expression in the transgenic lines relatively to the control; however, sucrose synthase 4 (SUSY4) was upregulated in transgenic tubers with AtGWD2 expression. Taken together, these results demonstrated that expression of AtGWD2 and AtGWD3 are not suitable for engineering starch with a high phosphate content in potato tubers but can provide new insights in the process of starch phosphorylation in the amyloplast.

KW - Glucan

KW - Granule morphology

KW - Starch metabolism

KW - Storage starch

KW - Transitory starch

KW - Water dikinase

U2 - 10.1002/star.201600324

DO - 10.1002/star.201600324

M3 - Article

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JO - Starch-Stärke

JF - Starch-Stärke

SN - 0038-9056

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