Heterologous expression of the pneumococcal serotype 14 polysaccharide in Lactococcus lactis requires lactococcal epsABC regulatory genes

M.N. Nierop Groot; J. Godefrooij; M. Kleerebezem

doi:10.1128/AEM.01655-07

Heterologous expression of the pneumococcal serotype 14 polysaccharide in Lactococcus lactis requires lactococcal epsABC regulatory genes

M.N. Nierop Groot, J. Godefrooij, M. Kleerebezem

Research output: Contribution to journal › Article › Academic › peer-review

6 Citations (Scopus)

Abstract

The pneumococcal serotype 14 polysaccharide was produced in Lactococcus lactis by coexpressing pneumococcal polysaccharide type 14-specific genes (cpsFGHIJKL(14)) with the lactococcal regulatory and priming glucosyltransferase-encoding genes specific for B40 polysaccharide (epsABCD(B40)). The polysaccharide produced by Lactococcus was secreted in the medium, simplifying downstream processing and polysaccharide isolation from culture broth

Original language	English
Pages (from-to)	912-915
Journal	Applied and Environmental Microbiology
Volume	74
Issue number	3
DOIs	https://doi.org/10.1128/AEM.01655-07
Publication status	Published - 2008

Keywords

streptococcus-pneumoniae
capsular polysaccharide
cell-wall
exopolysaccharides
length
cpsd

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title = "Heterologous expression of the pneumococcal serotype 14 polysaccharide in Lactococcus lactis requires lactococcal epsABC regulatory genes",

abstract = "The pneumococcal serotype 14 polysaccharide was produced in Lactococcus lactis by coexpressing pneumococcal polysaccharide type 14-specific genes (cpsFGHIJKL(14)) with the lactococcal regulatory and priming glucosyltransferase-encoding genes specific for B40 polysaccharide (epsABCD(B40)). The polysaccharide produced by Lactococcus was secreted in the medium, simplifying downstream processing and polysaccharide isolation from culture broth",

keywords = "streptococcus-pneumoniae, capsular polysaccharide, cell-wall, exopolysaccharides, length, cpsd",

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AU - Kleerebezem, M.

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N2 - The pneumococcal serotype 14 polysaccharide was produced in Lactococcus lactis by coexpressing pneumococcal polysaccharide type 14-specific genes (cpsFGHIJKL(14)) with the lactococcal regulatory and priming glucosyltransferase-encoding genes specific for B40 polysaccharide (epsABCD(B40)). The polysaccharide produced by Lactococcus was secreted in the medium, simplifying downstream processing and polysaccharide isolation from culture broth

AB - The pneumococcal serotype 14 polysaccharide was produced in Lactococcus lactis by coexpressing pneumococcal polysaccharide type 14-specific genes (cpsFGHIJKL(14)) with the lactococcal regulatory and priming glucosyltransferase-encoding genes specific for B40 polysaccharide (epsABCD(B40)). The polysaccharide produced by Lactococcus was secreted in the medium, simplifying downstream processing and polysaccharide isolation from culture broth

KW - streptococcus-pneumoniae

KW - capsular polysaccharide

KW - cell-wall

KW - exopolysaccharides

KW - length

KW - cpsd

U2 - 10.1128/AEM.01655-07

DO - 10.1128/AEM.01655-07

M3 - Article

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JO - Applied and Environmental Microbiology

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SN - 0099-2240

IS - 3

ER -

Heterologous expression of the pneumococcal serotype 14 polysaccharide in Lactococcus lactis requires lactococcal epsABC regulatory genes

Abstract

Keywords

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