Heterologous expression of the pneumococcal serotype 14 polysaccharide in Lactococcus lactis requires lactococcal epsABC regulatory genes

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Abstract

The pneumococcal serotype 14 polysaccharide was produced in Lactococcus lactis by coexpressing pneumococcal polysaccharide type 14-specific genes (cpsFGHIJKL(14)) with the lactococcal regulatory and priming glucosyltransferase-encoding genes specific for B40 polysaccharide (epsABCD(B40)). The polysaccharide produced by Lactococcus was secreted in the medium, simplifying downstream processing and polysaccharide isolation from culture broth
Original languageEnglish
Pages (from-to)912-915
JournalApplied and Environmental Microbiology
Volume74
Issue number3
DOIs
Publication statusPublished - 2008

Keywords

  • streptococcus-pneumoniae
  • capsular polysaccharide
  • cell-wall
  • exopolysaccharides
  • length
  • cpsd

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