Abstract
Itaconic acid, a C5-dicarboxylic acid, is a potential biobased building block for the polymer industry. It is obtained from the citric acid cycle by decarboxylation of cis-aconitic acid. This reaction is catalyzed by CadA in the native itaconic acid producer Aspergillus terreus. Recently, another enzyme encoded by the mammalian immunoresponsive gene 1 (irg1), was found to decarboxylate cis-aconitate to itaconate in vitro. We show that heterologous expression of irg1 enabled itaconate production in Escherichia coli with production titres up to 560 mg/L.
Original language | English |
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Article number | 849 |
Journal | Frontiers in Microbiology |
Volume | 6 |
DOIs | |
Publication status | Published - 2015 |
Keywords
- codon usage frequencies
- aspergillus-terreus
- acid production
- protein expression
- niger
- host