Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production

K.S. Vuoristo, A.E. Mars, S. Loon, E. Orsi, G. Eggink, J.P.M. Sanders, R.A. Weusthuis

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)

Abstract

Itaconic acid, a C5-dicarboxylic acid, is a potential biobased building block for the polymer industry. It is obtained from the citric acid cycle by decarboxylation of cis-aconitic acid. This reaction is catalyzed by CadA in the native itaconic acid producer Aspergillus terreus. Recently, another enzyme encoded by the mammalian immunoresponsive gene 1 (irg1), was found to decarboxylate cis-aconitate to itaconate in vitro. We show that heterologous expression of irg1 enabled itaconate production in Escherichia coli with production titres up to 560 mg/L.
Original languageEnglish
Article number849
JournalFrontiers in Microbiology
Volume6
DOIs
Publication statusPublished - 2015

Keywords

  • codon usage frequencies
  • aspergillus-terreus
  • acid production
  • protein expression
  • niger
  • host

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