Heterologous Expression and Purification of the CRISPR-Cas12a/Cpf1 Protein

Prarthana Mohanraju, John Oost, Martin Jinek, Daan Swarts

Research output: Contribution to journalArticleAcademicpeer-review


This protocol provides step by step instructions (Figure 1) for heterologous expression of Francisella novicida Cas12a (previously known as Cpf1) in Escherichia coli. It additionally includes a protocol for high-purity purification and briefly describes how activity assays can be performed. These protocols can also be used for purification of other Cas12a homologs and the purified proteins can be used for subsequent genome editing experiments.
Original languageEnglish
Issue number9
Publication statusPublished - 2018

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