Heme and menaquinone induced electron transport in lactic acid bacteria

R.J.W. Brooijmans, B. Smit, F. dos Santos, J. van Riel, W.M. de Vos, J. Hugenholtz

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Abstract

ABSTRACT: BACKGROUND: For some lactic acid bacteria higher biomass production as a result of aerobic respiration has been reported upon supplementation with heme and menaquinone. In this report, we have studied a large number of species among lactic acid bacteria for the existence of this trait. RESULTS: Heme- (and menaquinone) stimulated aerobic growth was observed for several species and genera of lactic acid bacteria. These include Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacilllus brevis, Lactobacillus paralimentarius, Streptococcus entericus and Lactococcus garviae. The increased biomass production without further acidification, which are respiration associated traits, are suitable for high-throughput screening as demonstrated by the screening of 8000 Lactococcus lactis insertion mutants. Respiration-negative insertion-mutants were found with noxA, bd-type cytochrome and menaquinol biosynthesis gene-disruptions. Phenotypic screening and in silico genome analysis suggest that respiration can be considered characteristic for certain species. CONCLUSION: We propose that the cyd-genes were present in the common ancestor of lactic acid bacteria, and that multiple gene-loss events best explains the observed distribution of these genes among the species
Original languageEnglish
Article number28
Number of pages11
JournalMicrobial Cell Factories
Volume8
DOIs
Publication statusPublished - 2009

Keywords

  • complete genome sequence
  • lactococcus-lactis
  • streptococcus-faecalis
  • respiration capacity
  • escherichia-coli
  • identification
  • cytochromes
  • generation
  • probiotics
  • alignment

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    Brooijmans, R. J. W., Smit, B., dos Santos, F., van Riel, J., de Vos, W. M., & Hugenholtz, J. (2009). Heme and menaquinone induced electron transport in lactic acid bacteria. Microbial Cell Factories, 8, [28]. https://doi.org/10.1186/1475-2859-8-28